Sequential Single Cell Transcriptional and Protein Marker Profiling Reveals TIGIT as a Marker of CD19 CAR-T Cell Dysfunction in Patients with Non-Hodgkin’s Lymphoma

To reveal changes in 4-1BB-based CD19 CAR-T cells and identify biomarkers of response, the authors employed single cell RNA sequencing and protein surface marker profiling of patient CAR-T cells pre- and post-infusion into non-Hodgkin’s lymphoma patients.