Optimized NGFR-Derived Hinges for Rapid and Efficient Enrichment and Detection of CAR T-cells In Vitro and In Vivo

Scientists reported the generation of two novel hinge domains derived from the low-affinity p75 chain of the human nerve growth factor receptor (NGFR), termed N3 and N4, which, when incorporated into the CAR backbone, allowed detection as well as high-grade enrichment of CAR T-cells with GMP-compatible immunomagnetic reagents.