Validation of a Rapid Potency Assay for Cord Blood Stem Cells Using Phospho Flow Cytometry: The IL-3-pSTAT5 Assay

Public cord blood banks are required to measure cord blood units potency before their release, thus scientists developed a rapid flow cytometry assay based on the measurement of STAT-5 phosphorylation of CD34+ stem cells in response to IL-3 stimulation.