A Bio-Orthogonal Linear Ubiquitin Probe Identifies STAT3 as a Direct Substrate of OTULIN in Glioblastoma

Investigators presented a live cell-compatible bio-orthogonal strategy to capture the direct linear polyUbiquitin-modified substrates. They constructed a linear Ubiquitin (Ub)-specific probe by internally incorporating a non-canonical amino acid into Ub via genetic code expansion.