Impaired AIF-CHCHD4 Interaction and Mitochondrial Calcium Overload Contribute to Auditory Neuropathy Spectrum Disorder in Patient-iPSC-Derived Neurons with AIFM1 Variant

Scientists generated induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells via nucleofection with episomal plasmids. The patient-specific iPSCs were edited via CRISPR/Cas9 technology to generate gene-corrected isogenic iPSCs.