High-Fat-Diet Induced Inflammation and Apoptosis via Activation of Ire1α in Liver and Hepatocytes of Black Seabream (Acanthopagrus schlegelii)

Primary hepatocytes were isolated from black seabream juveniles and treated with oleic acid (OA), OA + transfection with non-silencing control siRNA, and OA + transfection with ire1α-small interfering RNA for 48 hours versus untreated.