CRISPR-Cas9

[Stem Cell Research] To further understand the exact role of FLNC in dilated cardiomyopathy, scientists generated a human FLNC knockout cell line from the original ESC line H9 by CRISPR/Cas9 gene editing technology.

[Journal of Biological Chemistry] To better understand the role of TAF8 in TFIID, the authors investigated the requirement of the different regions of TAF8 for the in vitro assembly of lobe B and C, and the importance of certain TAF8 regions for mouse ESC viability.

[Hepatology International] Genetically modified organoids have been developed by sequential knockout and knockin of driver mutations by genome editing followed by niche-based selection.

[Scientific Reports] Scientists identified specific modulators of alpha synuclein gene (SNCA) transcription, generated CRISPR/Cas9 modified SNCA-GFP-luciferase genomic fusion- and control cell lines and screened a library of 1649 bioactive compounds, including the FDA approved drugs.

[Molecular Cancer Research] Researchers found that the expression of DCLK1 isoform 4 (DCLK1-S) was significantly increased in human esophageal squamous cell carcinoma tissues and associated with malignant progression and poor prognosis.

[Stem Cell Research] Scientists generated iPSCs from a normal individual by electroporation of peripheral blood mononuclear cells, and further generated a SCN1B-knockout human iPSC line via CRISPR/Cas9 gene editing.