embryonic stem cells

[Scientific Reports] Researchers optimized an homology-directed repair (HDR)-mediated knock-in method for mouse ESCs by improving the efficiency of HDR-mediated knock-in of a plasmid donor while almost completely suppressing non-homologous end joining/microhomology-mediated end joining-based integration.

[Scientific Reports] Scientists presented a transgenic, TAR DNA-binding protein-43 (TDP-43)-A315T, mouse model expressing an Amyotrophic Lateral Sclerosis (ALS) phenotype and demonstrated the presence of ubiquitinated cytoplasmic TDP-43 aggregates with > 80% cell death by 28 days post differentiation in vitro.

[Scientific Reports] Researchers presented a transgenic, TDP-43-A315T, mouse model expressing an amyotrophic lateral sclerosis phenotype and demonstrate the presence of ubiquitinated cytoplasmic TDP-43 aggregates with > 80% cell death by 28 days post differentiation in vitro.

[Critical Reviews in Biotechnology] Investigators review the use of human PSCs for skeletal tissue engineering, the different methods for characterization of skeletal cells and constructs at the tissue and single-cell level, and indicate newer resources not yet fully utilized in this field.

[Cell Stem Cell] Investigators showed an in vitro reconstitution of whole male germ-cell development by PSCs. Mouse ESCs were induced into primordial germ cell-like cells, which were expanded for epigenetic reprogramming.

[Cell Stem Cell] Scientists enhanced subcutaneous engraftment by employing a unique vascularization strategy based on ready-made microvessels isolated from the adipose tissue.