Efficient Biallelic Knock-In in Mouse Embryonic Stem Cells by In Vivo-Linearization of Donor and Transient Inhibition of DNA Polymerase θ/DNA-PK

Researchers optimized an homology-directed repair (HDR)-mediated knock-in method for mouse ESCs by improving the efficiency of HDR-mediated knock-in of a plasmid donor while almost completely suppressing non-homologous end joining/microhomology-mediated end joining-based integration.