Downregulation of E-cadherin in Pluripotent Stem Cells Triggers Partial EMT

Using a CRISPR interference system in human ESCs, scientists describe a molecular characterization of the effects of downregulation of E-cadherin, one of the main initiation events of EMT, as a unique start signal.
[Scientific Reports]
Aban, C. E., Lombardi, A., Neiman, G., Biani, M. C., La Greca, A., Waisman, A., Moro, L. N., Sevlever, G., Miriuka, S., & Luzzani, C. (2021). Downregulation of E-cadherin in pluripotent stem cells triggers partial EMT. Scientific Reports, 11(1), 2048. https://doi.org/10.1038/s41598-021-81735-1 Cite
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Direct Reprogramming of Epithelial Cell Rests of Malassez into Mesenchymal-Like Cells by Epigenetic Agents

Researchers examined the roles of 5-Azacytidine and valproic acid in the dedifferentiation of epithelial cell rests of Malassez into stem-like cells. Additionally, the ability of stem-like cells to differentiate into mesenchymal cells was evaluated.
[Scientific Reports]
Yoshida, K., Uehara, O., Kurashige, Y., Paudel, D., Onishi, A., Neopane, P., Hiraki, D., Morikawa, T., Harada, F., Takai, R., Sato, J., Saitoh, M., & Abiko, Y. (2021). Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents. Scientific Reports, 11(1), 1852. https://doi.org/10.1038/s41598-020-79426-4 Cite
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Direct Reprogramming of Epithelial Cell Rests of Malassez into Mesenchymal-Like Cells by Epigenetic Agents

Epithelial cell rests of malassez was cultured in ESC medium with 1 µM of 5-Azacytidine (5Aza), or 2 mM ofvalproic acid (Vpa), or a combination of 5Aza and Vpa. The cells stimulated with both 5Aza and Vpa were named as progenitor-dedifferentiated into stem-like cells.
[Scientific Reports]
Yoshida, K., Uehara, O., Kurashige, Y., Paudel, D., Onishi, A., Neopane, P., Hiraki, D., Morikawa, T., Harada, F., Takai, R., Sato, J., Saitoh, M., & Abiko, Y. (2021). Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents. Scientific Reports, 11(1), 1852. https://doi.org/10.1038/s41598-020-79426-4 Cite
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Great Expectations: Induced Pluripotent Stem Cell Technologies in Neurodevelopmental Impairments

Investigators introduce the development of induced pluripotent stem cells (iPSCs), the currently established iPSCs disease models, and artificial organoids related to neurodevelopmental impairments.
[International Journal of Medical Sciences]
Zhang, X., Li, Z., Liu, Y., & Gai, Z. (2021). Great Expectations: Induced pluripotent stem cell technologies in neurodevelopmental impairments. International Journal of Medical Sciences, 18(2), 459–473. https://doi.org/10.7150/ijms.51842 Cite
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The T-Box Transcription Factor Eomesodermin Governs Haemogenic Competence of Yolk Sac Mesodermal Progenitors

Investigators demonstrated using an ESC model that transient expression of the T-box transcription factor Eomesodermin governs haemogenic competency of extra-embryonic mesoderm.
[Nature Cell Biology]
Harland, L. T. G., Simon, C. S., Senft, A. D., Costello, I., Greder, L., Imaz-Rosshandler, I., Göttgens, B., Marioni, J. C., Bikoff, E. K., Porcher, C., de Bruijn, M. F. T. R., & Robertson, E. J. (2021). The T-box transcription factor Eomesodermin governs haemogenic competence of yolk sac mesodermal progenitors. Nature Cell Biology, 23(1), 61–74. https://doi.org/10.1038/s41556-020-00611-8 Cite
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m6A RNA Methylation Regulates the Fate of Endogenous Retroviruses

Using an unbiased genome-scale CRISPR knockout screen in mouse ESCs, investigators identified m6A RNA methylation as a way to restrict endogenous retroviruses.
[Nature]
Chelmicki, T., Roger, E., Teissandier, A., Dura, M., Bonneville, L., Rucli, S., Dossin, F., Fouassier, C., Lameiras, S., & Bourc’his, D. (2021). m 6 A RNA methylation regulates the fate of endogenous retroviruses. Nature, 1–5. https://doi.org/10.1038/s41586-020-03135-1 Cite
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Culture Surface Protein Coatings Affect the Barrier Properties and Calcium Signaling of hESC-RPE

Investigators identified the role of retinal pigment epithelium (RPE) basement membrane proteins type IV collagen, laminin, and nidogen-1 in the maturation and functionality of humn ESC-RPE after cryopreservation.
[Scientific Reports]
Viheriälä, T., Sorvari, J., Ihalainen, T. O., Mörö, A., Grönroos, P., Schlie-Wolter, S., Chichkov, B., Skottman, H., Nymark, S., & Ilmarinen, T. (2021). Culture surface protein coatings affect the barrier properties and calcium signalling of hESC-RPE. Scientific Reports, 11(1), 933. https://doi.org/10.1038/s41598-020-79638-8 Cite
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A Genetic Screen Identifies Etl4-Deficiency Capable of Stabilizing the Haploidy in Embryonic Stem Cells

The authors report a genome-wide loss-of-function screening that identified gene mutations that could significantly reduce the rate of self-diploidization in haploid ESCs.
[Stem Cell Reports]
Zhang, G., Li, X., Sun, Y., Wang, X., Liu, G., & Huang, Y. (2021). A Genetic Screen Identifies Etl4-Deficiency Capable of Stabilizing the Haploidy in Embryonic Stem Cells. Stem Cell Reports, 16(1), 29–38. https://doi.org/10.1016/j.stemcr.2020.11.016 Cite
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ERG1 Plays an Essential Role in Rat Cardiomyocyte Fate Decision by Mediating AKT Signaling

Using the rat embryonic stem cells model, investigators showed that ERG1 was crucial in cardiomyocyte lineage commitment via interactions with integrin β1.
[Stem Cells]
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The T-Box Transcription Factor Eomesodermin Governs Hemogenic Competence of Yolk Sac Mesodermal Progenitors

Investigators demonstrated using an embryonic stem cell model that transient expression of the T-box transcription factor Eomesodermin governed hemogenic competency of extra-embryonic mesoderm.
[Nature Cell Biology]
Harland, L. T. G., Simon, C. S., Senft, A. D., Costello, I., Greder, L., Imaz-Rosshandler, I., Göttgens, B., Marioni, J. C., Bikoff, E. K., Porcher, C., de Bruijn, M. F. T. R., & Robertson, E. J. (2021). The T-box transcription factor Eomesodermin governs haemogenic competence of yolk sac mesodermal progenitors. Nature Cell Biology, 23(1), 61–74. https://doi.org/10.1038/s41556-020-00611-8 Cite
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NRL -/- Gene Edited Human Embryonic Stem Cells Generate Rod-Deficient Retinal Organoids Enriched in S-Cone-Like Photoreceptors

Researchers used CRISPR/Cas9 gene editing to engineer an NRL-deficient embryonic stem cell line, and differentiated it into retinal organoids.
[Stem Cells]
NRL−/− gene edited human embryonic stem cells generate rod‐deficient retinal organoids enriched in S‐cone‐like photoreceptors - Cuevas - - STEM CELLS - Wiley Online Library. (n.d.). Retrieved January 8, 2021, from https://stemcellsjournals.onlinelibrary.wiley.com/doi/abs/10.1002/stem.3325?af=R Cite
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