AP-1 Is a Temporally Regulated Dual Gatekeeper of Reprogramming to Pluripotency

Scientists examined epigenome remodeling at the onset of human nuclear reprogramming by profiling human fibroblasts after fusion with murine ESCs. Efficient reprogramming of human fibroblasts to iPSCs was achieved by transduction with vectors expressing SOX2, KLF4, and inducible dnAP-1, demonstrating that dnAP-1 can substitute for exogenous human OCT4.
[Proceedings of the National Academy of Sciences of the United States of America]
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Highly Parallelized Human Embryonic Stem Cell Differentiation to Cardiac Mesoderm in Nanoliter Chambers on a Microfluidic Chip

Scientists differentiated human ESCs to early cardiac mesodermal cells in microfluidic chambers that had a volume of only 30 nanoliters, using discontinuous medium perfusion. To confirm cell pluripotency, they tracked hESC proliferation and immunostained the cells for pluripotency markers SOX2 and OCT3/4.
[Biomedical Microdevices]
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Emergent Synchronous Beating Behavior in Spontaneous Beating Cardiomyocyte Clusters

Researchers investigated the dominant rule determining synchronization of beating intervals of cardiomyocytes after the clustering of mouse primary and human embryonic stem cell-derived cardiomyocytes.
[Scientific Reports]
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Construction of a Mammalian Embryo Model from Stem Cells Organized by a Morphogen Signaling Centre

In situ hybridization, immunolabelling, cell tracking and transcriptomic analyses show that these embryoids form the three germ layers through a gastrulation process and that they exhibit a wide range of developmental structures, highly similar to neurula-stage mouse embryos.
[Nature Communications]
Xu, P.-F., Borges, R. M., Fillatre, J., de Oliveira-Melo, M., Cheng, T., Thisse, B., & Thisse, C. (2021). Construction of a mammalian embryo model from stem cells organized by a morphogen signalling centre. Nature Communications, 12(1), 3277. https://doi.org/10.1038/s41467-021-23653-4 Cite
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Ubiquitin-Specific Protease 3 Deubiquitinates and Stabilizes Oct4 Protein in Human Embryonic Stem Cells

Investigators generated a ubiquitin-specific protease 3 gene knockout using the CRISPR/Cas9 system and demonstrated that USP3 acts as a protein stabilizer of Oct4 by deubiquitinating Oct4.
[International Journal of Molecular Sciences]
Rhie, B.-H., Antao, A. M., Karapurkar, J. K., Kim, M.-S., Jo, W.-J., Ramakrishna, S., & Kim, K.-S. (2021). Ubiquitin-Specific Protease 3 Deubiquitinates and Stabilizes Oct4 Protein in Human Embryonic Stem Cells. International Journal of Molecular Sciences, 22(11), 5584. https://doi.org/10.3390/ijms22115584 Cite
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Induced Tissue-Specific Stem Cells (iTSCs): Their Generation and Possible Use in Regenerative Medicine

The authors provide a brief overview of iPSCs and discusses recent advances in the establishment of induced nduced tissue-specific stem cells and their possible applications in regenerative medicine.
[Pharmaceutics]
Pharmaceutics | Free Full-Text | Induced Tissue-Specific Stem Cells (iTSCs): Their Generation and Possible Use in Regenerative Medicine | HTML. (n.d.). Retrieved June 2, 2021, from https://www.mdpi.com/1999-4923/13/6/780/htm Cite
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Human Pluripotent Stem-Cell-Derived Models as a Missing Link in Drug Discovery and Development

By analyzing each stage of the drug discovery and development process, scientists identified the active role of human PSC-derived in vitro models in phenotypic screening, target-based screening, target validation, toxicology evaluation, precision medicine, clinical trial in a dish, and post-clinical studies.
[Pharmaceuticals]
Lin, X., Tang, J., & Lou, Y.-R. (2021). Human Pluripotent Stem-Cell-Derived Models as a Missing Link in Drug Discovery and Development. Pharmaceuticals, 14(6), 525. https://doi.org/10.3390/ph14060525 Cite
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Cross-Platform Validation of Neurotransmitter Release Impairments in Schizophrenia Patient-Derived NRXN1-Mutant Neurons

Scientists described a multicenter effort to test the generality of this pivotal observation, using independent analyses at two laboratories of patient-derived and newly engineered human neurons with heterozygous NRXN1 deletions.
[Proceedings of the National Academy of Sciences of the United States of America]
Pak, C., Danko, T., Mirabella, V. R., Wang, J., Liu, Y., Vangipuram, M., Grieder, S., Zhang, X., Ward, T., Huang, Y.-W. A., Jin, K., Dexheimer, P., Bardes, E., Mitelpunkt, A., Ma, J., McLachlan, M., Moore, J. C., Qu, P., Purmann, C., … Südhof, T. C. (2021). Cross-platform validation of neurotransmitter release impairments in schizophrenia patient-derived NRXN1-mutant neurons. Proceedings of the National Academy of Sciences, 118(22). https://doi.org/10.1073/pnas.2025598118 Cite
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Zbtb46-Dependent Altered Developmental Program in Embryonic Stem Cell-Derived Blood Cell Progenitors

Investigators probed the effect of the Zbtb46 transcription factor in ESC-derived blood cell progenitors using chemically inducible mouse cell lines.
[Stem Cells]
Boto, P., Gerzsenyi, T. B., Lengyel, A., Szunyog, B., & Szatmari, I. (n.d.). Zbtb46-dependent altered developmental program in embryonic stem cell-derived blood cell progenitors. STEM CELLS, n/a(n/a). https://doi.org/https://doi.org/10.1002/stem.3424 Cite
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Simplification of Culture Conditions and Feeder-Free Expansion of Bovine Embryonic Stem Cells

Simplified conditions will facilitate the use of bovine ESCs for gene editing applications and pluripotency and lineage commitment studies.
[Scientific Reports]
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Wnt–and Glutamate-Receptors Orchestrate Stem Cell Dynamics and Asymmetric Cell Division

Researchers discovered crosstalk between AMPA/Kainate-type ionotropic glutamate receptors and the Wnt-pathway during the initial Wnt3a-interaction at the cytonemes of mouse ESCs. In this study, they demonstrated that this crosstalk persisted throughout the Wnt3a-response in ESCs.
[eLife]
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