Modeling Human Retinoblastoma Using Embryonic Stem Cell-Derived Retinal Organoids

The authors present a protocol to establish a novel human retinoblastoma organoid model derived from genetically engineered human embryonic stem cells.
[STAR Protocols]
Liu, H., Hua, Z.-Q., & Jin, Z.-B. (2021). Modeling human retinoblastoma using embryonic stem cell-derived retinal organoids. STAR Protocols, 2(2), 100444. https://doi.org/10.1016/j.xpro.2021.100444 Cite
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Dissection of Two Routes to Naïve Pluripotency Using Different Kinase Inhibitors

Researchers use mass spectrometry to describe the molecular events triggered by inhibition of Mek1/2 and Gsk3 and inhibition of Cdk8/19 on ESCs.
[Nature Communications]
Martinez-Val, A., Lynch, C. J., Calvo, I., Ximénez-Embún, P., Garcia, F., Zarzuela, E., Serrano, M., & Munoz, J. (2021). Dissection of two routes to naïve pluripotency using different kinase inhibitors. Nature Communications, 12(1), 1863. https://doi.org/10.1038/s41467-021-22181-5 Cite
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Glycan Epitopes on 201B7 Human-Induced Pluripotent Stem Cells Using R-10G and R-17F Marker Antibodies

Investigators developed two hiPSC/hESC-specific glycan-recognizing mouse antibodies, R-10G and R-17F, using the Tic hiPSC line as an antigen. R-10G recognized a low-sulfate keratan sulfate, and R-17F recognized lacto-N-fucopentaose-1.
[Biomolecules]
Nagai, Y., Nakao, H., Kojima, A., Komatsubara, Y., Ohta, Y., Kawasaki, N., Kawasaki, N., Toyoda, H., & Kawasaki, T. (2021). Glycan Epitopes on 201B7 Human-Induced Pluripotent Stem Cells Using R-10G and R-17F Marker Antibodies. Biomolecules, 11(4), 508. https://doi.org/10.3390/biom11040508 Cite
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MYBL2 and ATM Suppress Replication Stress in Pluripotent Stem Cells

The authors proposed that in addition to ataxia‐telangiectasia and Rad3 related (ATR), a MYBL2‐MRN‐ATM replication stress response pathway functioned in ESCs to control DNA replication initiation and prevent genome instability.
[EMBO Reports]
Blakemore, D., Vilaplana-Lopera, N., Almaghrabi, R., Gonzalez, E., Moya, M., Ward, C., Murphy, G., Gambus, A., Petermann, E., Stewart, G. S., & García, P. (2021). MYBL2 and ATM suppress replication stress in pluripotent stem cells. EMBO Reports, n/a(n/a), e51120. https://doi.org/10.15252/embr.202051120 Cite
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Polyploid Giant Cancer Cells, Stemness and Epithelial-Mesenchymal Plasticity Elicited by Human Cytomegalovirus

Human cytomegalovirus presence paralleled the succession of the observed cellular and molecular events potentially ensuing the transformation process.
[Oncogene]
Nehme, Z., Pasquereau, S., Haidar Ahmad, S., Coaquette, A., Molimard, C., Monnien, F., Algros, M.-P., Adotevi, O., Diab Assaf, M., Feugeas, J.-P., & Herbein, G. (2021). Polyploid giant cancer cells, stemness and epithelial-mesenchymal plasticity elicited by human cytomegalovirus. Oncogene, 1–17. https://doi.org/10.1038/s41388-021-01715-7 Cite
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Isolation and Characterization of hESC-Derived Heart Field-Specific Cardiomyocytes Unravels New Insights into Their Transcriptional and Electrophysiological Profiles

Scientists described a high yield approach for isolation and characterization of hESC-derived heart field specific and nodal-like cardiomyocytes.
[Cardiovascular Research]
Isolation and characterization of hESC-derived heart field-specific cardiomyocytes unravels new insights into their transcriptional and electrophysiological profiles | Cardiovascular Research | Oxford Academic. (n.d.). Retrieved March 24, 2021, from https://academic.oup.com/cardiovascres/advance-article-abstract/doi/10.1093/cvr/cvab102/6179328?redirectedFrom=fulltext Cite
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Embryonic Stem Cell Derived Small Extracellular Vesicles Modulate Regulatory T Cells to Protect against Ischemic Stroke

Researchers determined the therapeutic function of embryonic stem cell derived extracellular vesicles, specifically focusing on their role in immunomodulation after ischemic stroke.
[ACS Nano]
Xia, Y., Hu, G., Chen, Y., Yuan, J., Zhang, J., Wang, S., Li, Q., Wang, Y., & Deng, Z. (2021). Embryonic Stem Cell Derived Small Extracellular Vesicles Modulate Regulatory T Cells to Protect against Ischemic Stroke. ACS Nano. https://doi.org/10.1021/acsnano.1c00672 Cite
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Efficient Induction of Neural Progenitor Cells from Human ESC/iPSCs on Type I Collagen

The authors established a novel and robust monolayer differentiation method to produce functional neural progenitor cells (NPC) from hESC/iPSCs on Type I Collagen. The derived cells not only displayed the requisite markers, but also behaved similarly to classic NPCs both in vitro and in vivo.
[Science China LIfe Sciences]
Liu, P., Chen, S., Wang, Y., Chen, X., Guo, Y., Liu, C., Wang, H., Zhao, Y., Wu, D., Shan, Y., Zhang, J., Wu, C., Li, D., Zhang, Y., Zhou, T., Chen, Y., Liu, X., Li, C., Wang, L., … Pei, D. (2021). Efficient induction of neural progenitor cells from human ESC/iPSCs on Type I Collagen. Science China Life Sciences. https://doi.org/10.1007/s11427-020-1897-0 Cite
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Generation of PARP1 Gene Knockout Human Embryonic Stem Cell Line Using CRISPR/Cas9

To clarify the function of PARP1 in hESCs, scientists report a PARP1 knockout hESC line, generated by CRISPR/Cas9 mediated gene targeting. This cell line showed normal karyotype, pluripotent stem cell marker expression and differentiation potential in vitro.
[Stem Cell Research]
Li, Y., Li, J., Zhou, T., Pan, G., & Huang, K. (2021). Generation of PARP1 gene knockout human embryonic stem cell line using CRISPR/Cas9. Stem Cell Research, 53, 102288. https://doi.org/10.1016/j.scr.2021.102288 Cite
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In Vivo Survival and Differentiation of Friedreich Ataxia iPSC‐Derived Sensory Neurons Transplanted in the Adult Dorsal Root Ganglia

To explore cell replacement therapies as a possible approach to treat Friedreich ataxia (FRDA), scientists examined transplantation of sensory neural progenitors derived from human embryonic stem cells and FRDA induced pluripotent stem cells (iPSC) into adult rodent dorsal root ganglia regions.
[Stem Cells Translational Medicine]
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Blastocyst-Like Structures Generated from Human Pluripotent Stem Cells

Starting from naive hiPSCs, investigators developed an effective three-dimensional culture strategy with successive lineage differentiation and self-organization to generate blastocyst-like structures in vitro.
[Nature]
Yu, L., Wei, Y., Duan, J., Schmitz, D. A., Sakurai, M., Wang, L., Wang, K., Zhao, S., Hon, G. C., & Wu, J. (2021). Blastocyst-like structures generated from human pluripotent stem cells. Nature, 1–7. https://doi.org/10.1038/s41586-021-03356-y Cite
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