Plexin-B2 Orchestrates Collective Stem Cell Dynamics via Actomyosin Contractility, Cytoskeletal Tension and Adhesion

Scientists showed a role of guidance receptor Plexin-B2 in organizing actomyosin network and adhesion complexes during multicellular development of human embryonic stem cells and neuroprogenitor cells.
[Nature Communications]
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Cell-Based Therapies for Retinal Diseases: A Review of Clinical Trials and Direct to Consumer “Cell Therapy” Clinics

Scientists outline the particular approaches in the different published clinical trials for cell-based therapies for retinal diseases, and present the controversies surrounding experimental treatments offered outside of legitimate studies.
[Stem Cell Research & Therapy]
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Regulation of NANOG and SOX2 Expression by Activin A and a Canonical WNT Agonist in Bovine Embryonic Stem Cells and Blastocysts

Activation of activin A signaling stimulated NANOG expression during self-renewal of bovine ESC but suppressed cells expressing pluripotency markers in the blastocyst and increased cells expressing CDX2.
[Biology Open]
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Generation of a Transgenic Mouse Embryonic Stem Cell Line Expressing Dnmt1Y495C Mutation Associated with HSAN1E Disorder

Researchers employed non-homologous recombination and generated a mouse ESC line carrying a transgene expressing DNMT1 Y495C mutation in the wild-type background as a model for studying the abnormal neurogenesis due to this mutation.
[Stem Cell Research]
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A CRISPR/Cas9 Strategy for the Generation of a FLNC Knockout hESC Line (WAe009-a-70) to Model Dilated Cardiomyopathy and Arrhythmogenic Right Ventricular Cardiomyopathy

To further understand the exact role of FLNC in dilated cardiomyopathy, scientists generated a human FLNC knockout cell line from the original ESC line H9 by CRISPR/Cas9 gene editing technology.
[Stem Cell Research]
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TAF8 Regions Important for TFIID Lobe B Assembly or for TAF2 Interactions Are Required for Embryonic Stem Cell Survival

To better understand the role of TAF8 in TFIID, the authors investigated the requirement of the different regions of TAF8 for the in vitro assembly of lobe B and C, and the importance of certain TAF8 regions for mouse ESC viability.
[Journal of Biological Chemistry]
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Joint Single-Cell Multiomic Analysis in Wnt3a Induced Asymmetric Stem Cell Division

Investigators developed an approach to jointly profile the epigenome and transcriptome in the same single cell. Utilizing this method, they profiled H3K27me3 and H3K4me3 levels along with gene expression in mouse ESCs with localized Wnt3a signaling, revealing the cell type-specific maps of the epigenome and transcriptome in divided daughter cells.
[Nature Communications]
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A Polycomb Domain Found in Committed Cells Impairs Differentiation When Introduced into PRC1 in Pluripotent Cells

ESCs expressing a chimeric CBX7 were impaired in their ability to properly form embryoid bodies and neural progenitor cells and showed reduced activation of lineage-specific genes across differentiation.
[Molecular Cell]
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Nuclear Transporter Importin-13 Plays a Key Role in the Oxidative Stress Transcriptional Response

Scientists performed transcriptomic analysis on wild-type and Importin-13-knockout mouse ESCs, revealing enrichment of differentially expressed genes involved in stress responses and apoptosis regulation.
[Nature Communications]
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Distinct Roles of Haspin in Stem Cell Division and Male Gametogenesis

Researchers showed that mouse ESCs that lacked or overexpressed haspin, albeit prone to chromosome misalignment during metaphase, could still divide, expand and differentiate.
[Scientific Reports]
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Loss of Resf1 Reduces the Efficiency of Embryonic Stem Cell Self-Renewal and Germline Entry

The authors used Resf1 knockout cell lines to determine the requirements of RESF1 for ESC self-renewal and for in vitro specification of ESCs into primordial germ cell-like cells
[Life Science Alliance]
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