induced pluripotent stem cells

[Science Advances] Scientists performed extensive single-cell transcriptomic analyses to map fate choices and gene expression patterns during hematopoietic differentiation of human PSCs and showed that oxidative metabolism was dysregulated during in vitro directed differentiation.

[Stem Cell Reviews and Reports] The chromatin accessibility and transcriptional level of human ESCs and human urine cells were compared by Assay for Transposase-Accessible Chromatin with high-throughput sequencing and RNA sequencing RNA-seq to identify potential reprogramming factors.

[Stem Cells] Using human ESCs as well as human iPSCs, investigators demonstrated that the level of reactive oxygen species in pluripotent cells oscillated in accordance with the cell cycle progression with the peak occurring at transition from S to G2/M phase of the cycle.

[Cell Reports] Researchers presented a human in vitro model permitting investigation of epithelial-intrinsic events culminating in AEC2 dysfunction, using patient-specific iPSCs carrying an AEC2-exclusive disease-associated variant (SFTPCI73T).

[Nature Communications] Comprehensively analyzing the mitochondrial genome in 146 iPSC and fibroblast lines from 151 donors, researchers showed that most age-related fibroblast mtDNA mutations were lost during reprogramming.

[eLife] The authors demonstrate that TGFβ signaling was required to maintain naïve human PSCs (hPSCs). The downstream effector proteins – SMAD2/3 – bound common sites in naïve and primed hPSCs, including shared pluripotency genes.