neural cells

[Communications Biology] Building on observations that mutations in the JIP3 gene result in lysosome-filled axonal swellings, scientists analyzed the impact of JIP3 depletion on the cytoskeleton of human neurons.

[Journal of Cell Science] Researchers showed that insulin secretion sites also contain non-neuronal proteins LL5β and KANK1, which in migrating cells organize exocytotic machinery in the vicinity of integrin-based adhesions.

[Nature Protocols] Scientists presented how to generate 3D spheroids resembling specific domains of the nervous system and then how to integrate them physically to allow axonal projections and synaptic assembly.

[Nature Protocols] The authors described an in vitro model of the human blood–brain barrier self-assembled within microfluidic devices from stem-cell-derived or primary brain endothelial cells, and primary brain pericytes and astrocytes.

[International Journal of Oncology] The authors evaluated the effects of tumor‑treating fields with different duty cycles on glioma spheroid cells and normal brain organoids.

[Nature Methods] Scientists established iTracer, a lineage recorder that combined reporter barcodes with inducible CRISPR–Cas9 scarring and was compatible with single-cell and spatial transcriptomics.