SHANK2 Mutations Impair Apoptosis, Proliferation and Neurite Outgrowth during Early Neuronal Differentiation in SH-SY5Y Cells

Using CRISPR/Cas9 genome editing, scientists obtaineded SH-SY5Y cell lines with frameshift mutations on one or both SHANK2 alleles. They investigated the effects of the different SHANK2 mutations on cell morphology, cell proliferation and differentiation potential during early neuronal differentiation.
[Scientific Reports]
Unsicker, C., Cristian, F.-B., von Hahn, M., Eckstein, V., Rappold, G. A., & Berkel, S. (2021). SHANK2 mutations impair apoptosis, proliferation and neurite outgrowth during early neuronal differentiation in SH-SY5Y cells. Scientific Reports, 11(1), 2128. https://doi.org/10.1038/s41598-021-81241-4 Cite
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Smoothened-Activating Lipids Drive Resistance to CDK4/6 Inhibition in Hedgehog-Associated Medulloblastoma Cells and Preclinical Models

Scientists identified resistance mechanisms to CDK6 inhibition in Hedgehog (HH)-associated medulloblastoma by performing orthogonal CRISPR and CRISPR interference screens in medulloblastoma cells treated with a CDK4/6 inhibitor, and RNA-sequencing of a mouse model of HH-associated medulloblastoma with genetic deletion of Cdk6.
[Journal of Clinical Investigation]
JCI - Smoothened-activating lipids drive resistance to CDK4/6 inhibition in Hedgehog-associated medulloblastoma cells and preclinical models. (n.d.). Retrieved January 22, 2021, from https://www.jci.org/articles/view/141171 Cite
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Phosphodiesterase Type-5 Inhibitor Tadalafil Modulates Steroid Hormones Signaling in a Prostate Cancer Cell Line

Researchers demonstrated for the first time that Tadalafil can significantly modulate androgen receptor expression and activity, Cyp19a1 and ERβ expression in prostate cancer cells, suggesting a specific effect of these proteins.
[International Journal of Molecular Sciences]
Bimonte, V. M., Marampon, F., Antonioni, A., Fittipaldi, S., Ferretti, E., Pestell, R. G., Curreli, M., Lenzi, A., Vitale, G., Brunetti, A., Migliaccio, S., & Aversa, A. (2021). Phosphodiesterase Type-5 Inhibitor Tadalafil Modulates Steroid Hormones Signaling in a Prostate Cancer Cell Line. International Journal of Molecular Sciences, 22(2), 754. https://doi.org/10.3390/ijms22020754 Cite
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Down Regulation of U2AF1 Promotes ARV7 Splicing and Prostate Cancer Progression

Scientists investigated the roles of U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1) in the resistance to anti-androgen treatment in prostate cancer and its underlying mechanism.
[Biochemical and Biophysical Research Communications]
Cao, H., Wang, D., Gao, R., Chen, L., & Feng, Y. (2021). Down regulation of U2AF1 promotes ARV7 splicing and prostate cancer progression. Biochemical and Biophysical Research Communications, 541, 56–62. https://doi.org/10.1016/j.bbrc.2020.12.111 Cite
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Energy Metabolism During Osteogenic Differentiation: The Role of Akt

Investigators tested the effect of osteogenic media containing ascorbate and β-glycerol phosphate, or various osteogenic hormones and growth factors on energy metabolism in long bone- and calvarial bone-derived osteoprogenitors.
[Stem Cells and Development]
Smith, C. O., & Eliseev, R. A. (2020). Energy Metabolism During Osteogenic Differentiation: The Role of Akt. Stem Cells and Development. https://doi.org/10.1089/scd.2020.0141 Cite
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Histone Demethylase KDM4C Controls Tumorigenesis of Glioblastoma by Epigenetically Regulating p53 and C-Myc

Researchers showed that KDM4C knockdown significantly repressed proliferation and tumorigenesis of glioblastoma cells in vitro and in vivo that were rescued by overexpressing wild-type KDM4C but not a catalytic dead mutant.
[Cell Death & Disease]
Lee, D. H., Kim, G. W., Yoo, J., Lee, S. W., Jeon, Y. H., Kim, S. Y., Kang, H. G., Kim, D.-H., Chun, K.-H., Choi, J., & Kwon, S. H. (2021). Histone demethylase KDM4C controls tumorigenesis of glioblastoma by epigenetically regulating p53 and c-Myc. Cell Death & Disease, 12(1), 1–14. https://doi.org/10.1038/s41419-020-03380-2 Cite
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LncRNA GAS6-AS2 Promotes Non-Small-Cell Lung Cancer Cell Proliferation via Regulating miR-144-3p/ MAPK6 Axis

GAS6-AS2 was identified, and its roles as well as mechanisms in regulating proliferation of non-small cell lung cancer cells were investigated.
[Cell Cycle]
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Regulation of Carcinogenesis and Mediation through Wnt/β-Catenin Signaling by 3,3′-Diindolylmethane in an Enzalutamide-Resistant Prostate Cancer Cell Line

To investigate whether combined treatment with Enzalutamide (ENZ) and 3,3′-diindolylmethane (DIM) could overcome ENZ resistance by regulating Wnt signaling to inhibit androgen receptor signaling and EMT in ENZ-resistant prostate cancer cells, 22Rv1 cells were cultured in normal medium and treated with ENZ, DIM, and DIM with ENZ.
[Scientific Reports]
Tsao, C.-W., Li, J.-S., Lin, Y.-W., Wu, S.-T., Cha, T.-L., & Liu, C.-Y. (2021). Regulation of carcinogenesis and mediation through Wnt/β-catenin signaling by 3,3′-diindolylmethane in an enzalutamide-resistant prostate cancer cell line. Scientific Reports, 11(1), 1239. https://doi.org/10.1038/s41598-020-80519-3 Cite
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MicroRNA-494-3p Inhibits Formation of Fast Oxidative Muscle Fibres by Targeting E1A-Binding Protein p300 in Human-Induced Pluripotent Stem Cells

Myogenesis in human induced pluripotent stem cells with the Tet/ON-myogenic differentiation 1 gene was induced by culturing them in doxycycline-supplemented differentiation medium for seven days.
[Scientific Reports]
Iwasaki, H., Ichihara, Y., Morino, K., Lemecha, M., Sugawara, L., Sawano, T., Miake, J., Sakurai, H., Nishi, E., Maegawa, H., & Imamura, T. (2021). MicroRNA-494-3p inhibits formation of fast oxidative muscle fibres by targeting E1A-binding protein p300 in human-induced pluripotent stem cells. Scientific Reports, 11(1), 1161. https://doi.org/10.1038/s41598-020-80742-y Cite
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HCMV-Mediated Interference of Bortezomib-Induced Apoptosis in Colon Carcinoma Cell Line Caco-2

Researchers investigated if proteasome inhibitors have therapeutic potential for colon carcinoma and how this is influenced by human cytomegalovirus (HCMV) infection. They showed by immunofluorescence and flow cytometry that the colon carcinoma cell line Caco-2 was susceptible to HCMV infection.
[Viruses-Basel]
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CTCF and EGR1 Suppress Breast Cancer Cell Migration through Transcriptional Control of Nm23-H1

Researchers examined the mechanisms that control Nm23-H1 expression in breast cancer cells.
[Scientific Reports]
Wong, K. M., Song, J., & Wong, Y. H. (2021). CTCF and EGR1 suppress breast cancer cell migration through transcriptional control of Nm23-H1. Scientific Reports, 11(1), 491. https://doi.org/10.1038/s41598-020-79869-9 Cite
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