Tag Archives: protein expression

Loss of Ephrin B2 Receptor (EPHB2) Sets Lipid Rheostat by Regulating Proteins DGAT1 and ATGL Inducing Lipid Droplet Storage in Prostate Cancer Cells

Knockdown strategies for EPHB2 were performed in prostate cancer cells to analyze the impact on the net lipid balance, proliferation, triacylglycerol-regulating proteins, effect on lipid droplet (LD) biogenesis, and intracellular localization of LDs.
[Laboratory Investigation]
Morales, A., Greenberg, M., Nardi, F., Gil, V., Hayward, S. W., Crawford, S. E., & Franco, O. E. (2021). Loss of ephrin B2 receptor (EPHB2) sets lipid rheostat by regulating proteins DGAT1 and ATGL inducing lipid droplet storage in prostate cancer cells. Laboratory Investigation, 1–14. https://doi.org/10.1038/s41374-021-00583-9 Cite
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RANK Signaling Increases after Anti-HER2 Therapy Contributing to the Emergence of Resistance in HER2-Positive Breast Cancer

Scientists showed that RANK bound to HER2 in breast cancer cells and that enhanced RANK pathway activation altered HER2 phosphorylation status.
[Breast Cancer Research]
Sanz-Moreno, A., Palomeras, S., Pedersen, K., Morancho, B., Pascual, T., Galván, P., Benítez, S., Gomez-Miragaya, J., Ciscar, M., Jimenez, M., Pernas, S., Petit, A., Soler-Monsó, M. T., Viñas, G., Alsaleem, M., Rakha, E. A., Green, A. R., Santamaria, P. G., Mulder, C., … Gonzalez-Suarez, E. (2021). RANK signaling increases after anti-HER2 therapy contributing to the emergence of resistance in HER2-positive breast cancer. Breast Cancer Research, 23(1), 42. https://doi.org/10.1186/s13058-021-01390-2 Cite
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Beneficial Effects of Human Umbilical Cord Blood Mononuclear Cells on Persistent Erectile Dysfunction After Treatment of 5-Alpha Reductase Inhibitor in Rats

In vivo erectile function, in vitro relaxant and contractile responses of CC, protein expression and localization of PDE5, eNOS, nNOS, HIF-1α, and smooth muscle content in penile tissue.
[Journal of Sexual Medicine]
Oztekin, C. V., Yilmaz-Oral, D., Kaya-Sezginer, E., Kirlangic, O. F., Ozen, F. Z., Ozdal, B., Topcu, H. O., & Gur, S. (2021). Beneficial Effects of Human Umbilical Cord Blood Mononuclear Cells on Persistent Erectile Dysfunction After Treatment of 5-Alpha Reductase Inhibitor in Rats. The Journal of Sexual Medicine, 0(0). https://doi.org/10.1016/j.jsxm.2021.02.005 Cite
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Lyophilized Powder of Mesenchymal Stem Cell Supernatant Attenuates Acute Lung Injury through the IL-6–P-STAT3–p63–JAG2 Pathway

Intratracheal bleomycin was used to induce acute lung injury in mice, and intratracheal MSCs in a supernatant lyophilized powder was administered as a treatment.
[Stem Cell Research & Therapy]
Peng, W., Chang, M., Wu, Y., Zhu, W., Tong, L., Zhang, G., Wang, Q., Liu, J., Zhu, X., Cheng, T., Li, Y., Chen, X., Weng, D., Liu, S., Zhang, H., Su, Y., Zhou, J., Li, H., & Song, Y. (2021). Lyophilized powder of mesenchymal stem cell supernatant attenuates acute lung injury through the IL-6–p-STAT3–p63–JAG2 pathway. Stem Cell Research & Therapy, 12(1), 216. https://doi.org/10.1186/s13287-021-02276-y Cite
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CircRNA RSF1 Regulated Ox-LDL Induced Vascular Endothelial Cells Proliferation, Apoptosis and Inflammation through Modulating miR-135b-5p/HDAC1 Axis in Atherosclerosis

ox-LDL inhibited circRSF1 and histone deacetylase 1 (HDAC1) expression while upregulated miR-135b-5p expression in HUVECs.
[Biological Research]
Zhang, X., Lu, J., Zhang, Q., Luo, Q., & Liu, B. (2021). CircRNA RSF1 regulated ox-LDL induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in atherosclerosis. Biological Research, 54(1), 11. https://doi.org/10.1186/s40659-021-00335-5 Cite
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Colon Epithelial Cell TGFβ Signaling Modulates the Expression of Tight Junction Proteins and Barrier Function in Mice

In vitro colon organoid culture was utilized to assess junction-related gene expression by qPCR and trans-epithelial resistance. In silico analyses of human inflammatory bowel disease and colon cancer databases were performed.
[American Journal of Physiology-Gastrointestinal and Liver Physiology]
Marincola Smith, P., Choksi, Y. A., Markham, N. O., Hanna, D. N., Zi, J., Weaver, C. J., Hamaamen, J. A., Lewis, K. B., Yang, J., Liu, Q., Kaji, I., Means, A. L., & Beauchamp, R. D. (2021). Colon epithelial cell TGFβ signaling modulates the expression of tight junction proteins and barrier function in mice. American Journal of Physiology-Gastrointestinal and Liver Physiology. https://doi.org/10.1152/ajpgi.00053.2021 Cite
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S-Adenosylmethionine Upregulates the Angiotensin Receptor-Binding Protein ATRAP via the Methylation of HuR in NAFLD

The authors explored the effect of supplementary S-adenosylmethionine, which was the main biological methyl donor in mammalian cells, in regulating AT1R-associated protein (ATRAP), which was the negative regulator of AT1R.
[Cell Death & Disease]
Guo, T., Dai, Z., You, K., Battaglia-Hsu, S.-F., Feng, J., Wang, F., Li, B., Yang, J., & Li, Z. (2021). S-adenosylmethionine upregulates the angiotensin receptor-binding protein ATRAP via the methylation of HuR in NAFLD. Cell Death & Disease, 12(4), 1–12. https://doi.org/10.1038/s41419-021-03591-1 Cite
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Interferon Regulatory Factor 1 (IRF-1) Downregulates Checkpoint Kinase 1 (CHK1) through miR-195 to Upregulate Apoptosis and PD-L1 Expression in Hepatocellular Carcinoma (HCC) Cells

IRF-1 induces miR-195 to suppress CHK1 protein expression. Both increased IRF-1 and decreased CHK1 upregulate cellular apoptosis and PD-L1 expression in HCC.
[British Journal of Cancer]
Yan, Y., Zheng, L., Du, Q., Cui, X., Dong, K., Guo, Y., & Geller, D. A. (2021). Interferon regulatory factor 1 (IRF-1) downregulates Checkpoint kinase 1 (CHK1) through miR-195 to upregulate apoptosis and PD-L1 expression in Hepatocellular carcinoma (HCC) cells. British Journal of Cancer, 1–11. https://doi.org/10.1038/s41416-021-01337-6 Cite
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Investigating Heterogeneities of Live Mesenchymal Stromal Cells Using AI-Based Label-Free Imaging

The authors developed an artificial intelligence (AI)-based method that converts transmitted light microscopy images of MSCs into quantitative measurements of protein expression levels.
[Scientific Reports]
Imboden, S., Liu, X., Lee, B. S., Payne, M. C., Hsieh, C.-J., & Lin, N. Y. C. (2021). Investigating heterogeneities of live mesenchymal stromal cells using AI-based label-free imaging. Scientific Reports, 11(1), 6728. https://doi.org/10.1038/s41598-021-85905-z Cite
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SARS-CoV-2 Infection of the Oral Cavity and Saliva

Saliva from SARS-CoV-2-infected individuals harbored epithelial cells exhibiting ACE2 and TMPRSS expression and sustained SARS-CoV-2 infection.
[Nature Medicine]
Huang, N., Pérez, P., Kato, T., Mikami, Y., Okuda, K., Gilmore, R. C., Conde, C. D., Gasmi, B., Stein, S., Beach, M., Pelayo, E., Maldonado, J. O., Lafont, B. A., Jang, S.-I., Nasir, N., Padilla, R. J., Murrah, V. A., Maile, R., Lovell, W., … Byrd, K. M. (2021). SARS-CoV-2 infection of the oral cavity and saliva. Nature Medicine, 1–12. https://doi.org/10.1038/s41591-021-01296-8 Cite
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Long QT Syndrome 2 PAS Domain Variant Induces hERG1a/1b Subunit Imbalance in Patient-Specific iPSC-Cardiomyocytes

hiPSCs were derived from a long QT syndrome type 2 (LQT2) patient carrying the Per-Arnt-Sim (PAS) domain variant hERG1-H70R. CRISPR/Cas9 gene editing produced isogenic control iPSC lines. Differentiated iPSC-derived cardiomyocytes were evaluated for their electrophysiology, hERG1a/1b mRNA expression, and hERG1a/1b protein expression.
[Circulation-Arrhythmia and Electrophysiology]
Feng Li, Zhang Jianhua, Lee ChangHwan, Kim Gina, Liu Fang, Petersen Andrew J., Lim Evi, Anderson Corey L., Orland Kate M., Robertson Gail A., Eckhardt Lee L., January Craig T., & Kamp Timothy J. (n.d.). Long QT Syndrome 2 PAS Domain Variant Induces hERG1a/1b Subunit Imbalance in Patient-specific iPSC-cardiomyocytes. Circulation: Arrhythmia and Electrophysiology, 0(0). https://doi.org/10.1161/CIRCEP.120.009343 Cite
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