Using CRISPR/Cas9 genome editing, scientists obtaineded SH-SY5Y cell lines with frameshift mutations on one or both SHANK2 alleles. They investigated the effects of the different SHANK2 mutations on cell morphology, cell proliferation and differentiation potential during early neuronal differentiation.
6807162 SYHHCIUK items 1 apa default asc 1
Unsicker, C., Cristian, F.-B., von Hahn, M., Eckstein, V., Rappold, G. A., & Berkel, S. (2021). SHANK2 mutations impair apoptosis, proliferation and neurite outgrowth during early neuronal differentiation in SH-SY5Y cells. Scientific Reports, 11(1), 2128. https://doi.org/10.1038/s41598-021-81241-4 Cite
Scientists identified resistance mechanisms to CDK6 inhibition in Hedgehog (HH)-associated medulloblastoma by performing orthogonal CRISPR and CRISPR interference screens in medulloblastoma cells treated with a CDK4/6 inhibitor, and RNA-sequencing of a mouse model of HH-associated medulloblastoma with genetic deletion of Cdk6.
[Journal of Clinical Investigation]
Researchers demonstrated for the first time that Tadalafil can significantly modulate androgen receptor expression and activity, Cyp19a1 and ERβ expression in prostate cancer cells, suggesting a specific effect of these proteins.
[International Journal of Molecular Sciences]
6630899 LH95REZX items 1 apa default asc 1
Bimonte, V. M., Marampon, F., Antonioni, A., Fittipaldi, S., Ferretti, E., Pestell, R. G., Curreli, M., Lenzi, A., Vitale, G., Brunetti, A., Migliaccio, S., & Aversa, A. (2021). Phosphodiesterase Type-5 Inhibitor Tadalafil Modulates Steroid Hormones Signaling in a Prostate Cancer Cell Line. International Journal of Molecular Sciences, 22(2), 754. https://doi.org/10.3390/ijms22020754 Cite
Scientists investigated the roles of U2 Small Nuclear RNA Auxiliary Factor 1 (U2AF1) in the resistance to anti-androgen treatment in prostate cancer and its underlying mechanism.
[Biochemical and Biophysical Research Communications]
Investigators tested the effect of osteogenic media containing ascorbate and β-glycerol phosphate, or various osteogenic hormones and growth factors on energy metabolism in long bone- and calvarial bone-derived osteoprogenitors.
[Stem Cells and Development]
Researchers showed that KDM4C knockdown significantly repressed proliferation and tumorigenesis of glioblastoma cells in vitro and in vivo that were rescued by overexpressing wild-type KDM4C but not a catalytic dead mutant.
[Cell Death & Disease]
6807162 BNZ6R9PW items 1 apa default asc 1
Lee, D. H., Kim, G. W., Yoo, J., Lee, S. W., Jeon, Y. H., Kim, S. Y., Kang, H. G., Kim, D.-H., Chun, K.-H., Choi, J., & Kwon, S. H. (2021). Histone demethylase KDM4C controls tumorigenesis of glioblastoma by epigenetically regulating p53 and c-Myc. Cell Death & Disease, 12(1), 1–14. https://doi.org/10.1038/s41419-020-03380-2 Cite
GAS6-AS2 was identified, and its roles as well as mechanisms in regulating proliferation of non-small cell lung cancer cells were investigated.
1254621 PHLFEB2K items 1 apa default asc 1
To investigate whether combined treatment with Enzalutamide (ENZ) and 3,3′-diindolylmethane (DIM) could overcome ENZ resistance by regulating Wnt signaling to inhibit androgen receptor signaling and EMT in ENZ-resistant prostate cancer cells, 22Rv1 cells were cultured in normal medium and treated with ENZ, DIM, and DIM with ENZ.
6630899 ZPCG49Z6 items 1 apa default asc 1
Tsao, C.-W., Li, J.-S., Lin, Y.-W., Wu, S.-T., Cha, T.-L., & Liu, C.-Y. (2021). Regulation of carcinogenesis and mediation through Wnt/β-catenin signaling by 3,3′-diindolylmethane in an enzalutamide-resistant prostate cancer cell line. Scientific Reports, 11(1), 1239. https://doi.org/10.1038/s41598-020-80519-3 Cite
Myogenesis in human induced pluripotent stem cells with the Tet/ON-myogenic differentiation 1 gene was induced by culturing them in doxycycline-supplemented differentiation medium for seven days.
1254621 UA63RZ5D items 1 apa default asc 1
Iwasaki, H., Ichihara, Y., Morino, K., Lemecha, M., Sugawara, L., Sawano, T., Miake, J., Sakurai, H., Nishi, E., Maegawa, H., & Imamura, T. (2021). MicroRNA-494-3p inhibits formation of fast oxidative muscle fibres by targeting E1A-binding protein p300 in human-induced pluripotent stem cells. Scientific Reports, 11(1), 1161. https://doi.org/10.1038/s41598-020-80742-y Cite
Researchers investigated if proteasome inhibitors have therapeutic potential for colon carcinoma and how this is influenced by human cytomegalovirus (HCMV) infection. They showed by immunofluorescence and flow cytometry that the colon carcinoma cell line Caco-2 was susceptible to HCMV infection.
1254621 5L4I7UDL items 1 apa default asc 1
Researchers examined the mechanisms that control Nm23-H1 expression in breast cancer cells.