qRT-PCR

[Scientific Reports] Investigators explored the regulatory mechanism of circFOXM1 in hepatocellular carcinoma (HCC) proliferation and metastasis. RNA polymerase inhibitor actinomycin D and RNase R exonuclease were used to identify circFOXM1 in HCC cells.

[Journal of Experimental & Clinical Cancer Research] Scientists modulated the expression of ROR2 in human breast cancer cells and characterized their gene and protein expression by RNA-Seq, qRT-PCR, immunoblots and reverse phase protein array combined with network analyses to understand the molecular basis of ROR2 signaling in breast cancer.

[Experimental Eye Research] The retinoblastoma stem-like cells were isolated by single cell cloning in combination of examination of sphere-forming capacities.

[Bioengineered] Exosomes were extracted from the supernatant of the bone-marrow MSCs. The expression of the micro-RNA miR-93-3p was determined by qRT-PCR analysis.

[Circulation Journal] The expression of nuclear paraspeckle assembly transcript 1, miR-30d-5p and A disintegrin and metalloprotease 10 was measured by qRT-PCR and western blot. Functional experiments were conducted by using a CCK-8 assay, EDU assay, flow cytometry, western blot, ELISA, and commercial kits.

[Bioengineered] The underlying molecular mechanisms of PSP in improving insulin resistance (IR) in skeletal muscle remain unclear. PA induced an IR model in L6 myotubes. After treatment, cell proliferation was measured using the CCK8.