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RhoA/ROCK1 Regulates the Mitochondrial Dysfunction through Drp1 Induced by Porphyromonas gingivalis in Endothelial Cells

[Journal of Cellular and Molecular Medicine] The authors investigated the role of the Ras homologous gene family member A (RhoA)/Rho-related coiled-coil containing protein kinase (ROCK1) pathway in regulating mitochondrial dysfunction caused by P. gingivalis. P. gingivalis was used to infect EA.hy926 cells.

Direct Contact with Endothelial Cells Drives Dental Pulp Stem Cells Toward Smooth Muscle Cells Differentiation via TGF-β1 Secretion

[International Endodontic Journal] HUVECs and dental pulp stem cells were directly cocultured or indirectly cocultured with transwell inserts in endothelial basal media-2 supplemented with 5% FBS for six days.

Bioinspired Engineering of Fusogen and Targeting Moiety Equipped Nanovesicles

[Nature Communications] Researchers reported a bioinspired material, engineered fusogen and targeting moiety co-functionalized cell-derived nanovesicle (CNV) called eFT-CNV, as a drug vehicle.

Generation and Purification of ACTH-Secreting hPSC-Derived Pituitary Cells for Effective Transplantation

[Stem Cell Reports] Building on development of self-organizing culture to generate pituitary-hypothalamic organoids (PHOs) using human pluripotent stem cells (hPSCs), the authors established techniques to generate PHOs using feeder-free hPSCs and to purify pituitary cells.

In-Vivo Programmable Acoustic Manipulation of Genetically Engineered Bacteria

[Nature Communications] Investigators used the heterologous expression of gene clusters to generate genetically engineered bacteria that could produce numerous sub-micron gas vesicles in the bacterial cytoplasm.

Mesenchymal Properties of iPSC-Derived Neural Progenitors That Generate Undesired Grafts after Transplantation

[Communications Biology] Researchers established single cell-derived neural stem/progenitor cells clones (scNS/PCs) from human induced pluripotent stem cells (hiPSC)-NS/PCs that generated undesired grafts.

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