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Generation of Human Endometrial Assembloids with a Luminal Epithelium Using Air–Liquid Interface Culture Methods

[Advanced Science] A novel endometrial assembloid model was developed by combining endometrial epithelial cells and stromal cells, and using an improved matrix and air–liquid interface culture method.

The Mechano-Chemical Circuit Drives Skin Organoid Self-Organization

[Proceedings Of The National Academy Of Sciences Of The United States Of America] Cell behavior videos showed that the morphological transformation from multiple spheroidal units with morphogenesis competence to planar skin was characterized by two abrupt cell motility–increasing events before calming down.

A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis

[Advanced Healthcare Materials] The authors engineered “microarray three-dimensional bioprinting” technology and associated pillar and perfusion plates for human organoid culture and analysis.

Druggable Redox Pathways against Mycobacterium abscessus in Cystic Fibrosis Patient-Derived Airway Organoids

[PLOS Pathogens] Investigators used human airway organoids microinjected with smooth or rough Mycobacterium abscessus to evaluate bacteria fitness, host responses to infection, and new treatment efficacy.

Microfluidic Droplet Encapsulation-Guided Organoid Growth Promotes Parental Tumor Phenotype Recapitulation

[International Journal of Cancer] Researchers reported a method of microfluidic droplet encapsulation that provided structural guidance for tumor cell growth and organization, where they developed into tumor organoids with high purity and high rates of modeling success.

Pancreatic Duct Organoid Swelling Is Chloride-Dependent

[Journal of Cystic Fibrosis] Scientists generated pancreatic duct organoids from Cystic fibrosis (CF) and non-CF pigs. Epithelial and ductal origin was confirmed with epithelial markers, ion transporters, and lack of acinar, islet cell markers.

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