Enhanced Cortical Neural Stem Cell Identity through Short SMAD and WNT Inhibition in Human Cerebral Organoids Facilitates Emergence of Outer Radial Glial Cells

Researchers showed that a short and early Dual SMAD and WNT inhibition course was necessary and sufficient to establish robust and lasting cortical organoid neural stem cells (NSC) identity, efficiently suppressing non-cortical NSC fates.