Meiosis Resumption in Human Primordial Germ Cells from Induced Pluripotent Stem Cells by In Vitro Activation and Reconstruction of Ovarian Nests

iPSCs carrying a SYCP3-mkate2 knock-in reporter were generated by the CRISPR/Cas9 strategy to monitor meiosis status during induced differentiation from iPSCs into oocyte.