Generation of a Homozygous and a Heterozygous SNCA Gene Knockout Human-Induced Pluripotent Stem Cell Line by CRISPR/Cas9 Mediated Allele-Specific Tuning of SNCA Expression

Researchers developed two hiPSC lines using CRISPR/Cas9-mediated allele-specific frame-shift deletion of the aSyn encoding gene SNCA, resulting in homo- and heterozygous SNCA knockout.