Transient Inhibition of p53 Enhances Prime Editing and Cytosine Base-Editing Efficiencies in Human Pluripotent Stem Cells

Scientists reported that co-delivery of p53DD, a dominant negative fragment of p53, can greatly enhance prime editing (PE) and cytosine base editing efficiencies in generating precise mutations in hPSCs. They further applied PE3 enzyme in combination with p53DD to efficiently create multiple isogenic hPSC lines.