Broadly Applicable Hydrogel Fabrication Procedures Guided by YAP/TAZ-Activity Reveal Stiffness, Adhesiveness, and Nuclear Projected Area as Checkpoints for Mechanosensing

Protocols were streamlined for the synthesis and functionalization of cell adhesive polyacrylamide-based and fully-defined polyethyleneglycol-based hydrogels tuned at various rigidities for mechanobiology experiments, from 0.3 to >10 kPa.