Efficient Simultaneous Double DNA Knock-In in Murine Embryonic Stem Cells by CRISPR/Cas9 Ribonucleoprotein-Mediated Circular Plasmid Targeting for Generating Gene-Manipulated Mice

Researchers first compared the KI efficiency of mESCs with CRISPR/Cas9 expression vector and ribonucleoprotein (RNP), and confirmed that KI efficiency was significantly increased by using RNP.