Single-Cell RNA Sequencing and Spatial Transcriptomics Reveal Cancer-Associated Fibroblasts in Glioblastoma with Protumoral Effects

Cells without copy number variations and with high cancer-associated fibroblasts (CAF) probability scores were identified in single-cell RNA-Seq of 12 patient glioblastomas. Pseudotime reconstruction revealed that immature CAFs evolved into subtypes, with mature CAFs expressing actin alpha 2, smooth muscle