Isolation and Identification of Extracellular Matrix Proteins from Oil-Based CASPERized Mouse Brains for Matrisomal Analysis

Researchers immersed mouse brains in oil for polymerization and decellularization via Oil-based Clinically and Experimentally Applicable Acellular Tissue Scaffold Production for Tissue Engineering and Regenerative Medicine and then isolated ECM components using sequential matrisome preparation reagents, namely, RIPA, PNGase F, and concanavalin A.