Rif1 Regulates Self-Renewal and Impedes Mesendodermal Differentiation of Mouse Embryonic Stem Cells

Investigators generated a Rif1 conditional knockout mESC line based on Cre-loxP system. Western blot, flow cytometry, qRT-PCR, RNA-Seq, ChIP-Seq, ChIP-qPCR, immunofluorescence, and immunoprecipitation were employed for phenotype and molecular mechanism assessment.