Adjustable Extracellular Matrix Rigidity Tumor Model for Studying Stiffness Dependent Pancreatic Ductal Adenocarcinomas Progression and Tumor Immunosuppression

A gelatin methacryloyl (GelMA)-based hydrogel was designed for in vitro and in vivo PDAC experiments.The GelMA-based in vitro 3D culture method could effectively form a gradient and stable extracellular matrix stiffness.