Human iPSC-Derived Proinflammatory Macrophages Cause Insulin Resistance in An Isogenic White Adipose Tissue Microphysiological System

Human induced pluripotent stem cell derived macrophages (iMACs) and adipocytes (iADIPOs) were cocultured in a microphysiological system. iMACs migrated toward and infiltrated into the 3D iADIPOs cluster to form crown-like structures-like morphology around damaged iADIPOs, recreating classic histological features of white adipose tissue inflammation seen in obesity.