Enhancement of CAR-T Cell Activity against Cholangiocarcinoma by Simultaneous Knockdown of Six Inhibitory Membrane Proteins

Researchers knocked down immune checkpoints and immunosuppressive molecular receptors in CAR-T cells by constructing two clusters of small hairpin RNAs and evaluated the engineered CAR-T cells for antitumor activity both in vitro, using tumor cell lines and cholangiocarcinoma organoid models, and in vivo, using humanized mouse models.