Cell Softness Reveals Tumorigenic Potential via ITGB8/AKT/Glycolysis Signaling in a Mice Model of Orthotopic Bladder Cancer

The stiffness of bladder cancer cells was determined by atomic force microscopy (AFM). The modified microfluidic chip was utilized to separate soft cells, and the 3D Matrigel culture system was to maintain the softness of tumor cells.