Substrate Stiffness Can Affect the Crosstalk Between Adipose Derived Mesenchymal Stem Cells and Macrophages in Bone Tissue Engineering

Biomaterials with Young’s modulus of 64 and 0.2 kPa were selected, and the crosstalk between adipose-derived MSCSs (ADSCs) and macrophages was investigated by means of conditioned medium treatment and cell co-culture, respectively. PCR and flow cytometry were used to evaluate the polarization of macrophages.