Silymarin Prevents Endothelial Dysfunction by Upregulating Erk-5 in Oxidized LDL Exposed Endothelial Cells

Researchers examined the effects of silymarin in regulating the expression of Erk-5 and ameliorating endothelial dysfunction using in vitro and in vivo models. Primary human umbilical vascular endothelial cells viability was measured by MTT assay; mRNA and protein expression by RT-qPCR and Western blotting; tube-formation assay was performed to examine endothelialness.