Prolonged Culturing of Colonic Epithelial Organoids Derived from Healthy Individuals and Ulcerative Colitis Patients Results in the Decrease of LINE-1 Methylation Level

Researchers reported that long-term culturing of colonic epithelial organoids generated from stem cells of healthy and diseased individuals resulted in decreased nucleotide element 1 (LINE-1) methylation level, when compared to tissue of origin and short-term cultures.