Mesenchymal Stem/Stromal Cells From Human Pluripotent Stem Cell-Derived Brain Organoid Enhance the Ex Vivo Expansion and Maintenance of Hematopoietic Stem/Progenitor Cells

Flow cytometric analysis, RNA sequencing and differentiation ability were applied to detect the characteristics of stromal cells from 3D human brain organoids. Human umbilical cord blood CD34+ cells were cultured in different coculture conditions composed of stromal cells and umbilical cord MSCs (UC-MSCs) with or without a cytokine cocktail.