Adipose Tissue-Derived Mesenchymal Stem Cell Inhibits Osteoclast Differentiation through Tumor Necrosis Factor Stimulated Gene-6

Human adipose tissue-derived (AD) MSCs were co-cultured with bone marrow-derived monocyte/macrophage from DBA/1J or B6 mouse in the presence of osteoclastogenic condition. In some co-culture groups, ADMSCs were transfected with siRNA targeting TSG-6 or osteoprotegerin to determine their role in osteoclastogenesis.