Live-Attenuated Virus Vaccine Defective in RNAi Suppression Induces Rapid Protection in Neonatal and Adult Mice Lacking Mature B and T Cells

The mosquito-transmitted Nodamura virus was attenuated in mice by mutations that prevent expression of the B2 viral suppressor of RNA interference and consequently, drastically enhance in vivo production of the virus-targeting small-interfering RNAs.