TBC1D30 Regulates Proinsulin and Insulin Secretion and Is the Target of a Genomic Association Signal for Proinsulin

Using CRISPR/Cas9 genome editing to create double-strand breaks and prime editing to install substitutions in INS1 832/13 insulinoma cells, scientists generated clonal cell lines with altered TBC1D30, as well as homozygous and heterozygous lines carrying the lead GWAS variant.