Hanging Drop Culture Reprograms Mesenchymal Stem Cell Transcriptome to Enhance Cell Delivery Efficiency via Attenuated Pulmonary Entrapment

Scientists utilized a hanging drop method to generate 3D spheroid-derived MSCs and compared them to conventionally 2D-cultured MSCs. The results showed that 3D MSCs exhibited distinct phenotypic features than 2D-cultured MSCs and expressed different transcriptional responses, as found from RNA-Seq analysis.