Umbilical & Placental Cell News
A Pluripotent Stem Cell Atlas of Multilineage Differentiation
[Scientific Data] Researchers generated a single-cell RNA sequencing time course to study the role of developmental signaling pathways on multilineage diversification from pluripotency in vitro.
DNA Aptamer-Guided Glycomimetics for Developmental Stage-Specific Glycocalyx Engineering to Control Stem Cell Differentiation
[Journal of The American Chemical Society] Scientists reported a heparan sulfate mimetic containing a DNA aptamer with affinity for the membrane-associated pluripotency marker, alkaline phosphatase, that can be selectively targeted to the surface of ESCs.
Human Neuron Subtype Programming via Single-Cell Transcriptome-Coupled Patterning Screens
[Science] Investigators modulated developmental signaling pathways combined with transcripiton factor overexpression to explore the spectrum of neuron subtypes generated from PSCs.
Analysis of a BCOR Internal Tandem Duplication in Mouse Embryonic Stem Cell to Neuronal Precursor Differentiation
[Genetics] To dissect the molecular mechanisms underlying aberrant function of BCL-6 corepressor (BCOR)-internal tandem duplication (ITD) mutants, scientists employed mESCs expressing either transgenic or endogenous BCOR-ITD.
Mutational Landscape of Pure Ductal Carcinoma In Situ and Associations with Disease Prognosis and Response to Radiotherapy
[Breast Cancer Research] Through whole-exome sequencing of 147 ductal carcinoma in situ (DCIS) cases, scientists characterized the genomic landscape of pure DCIS and identified genetic alterations associated with the risk of recurrence.
DHX9-Mediated Epigenetic Silencing of BECN1 Contributes to Impaired Autophagy and Tumor Progression in Breast Cancer via Recruitment of HDAC5
[Cell Death & Disease] Scientists found that DHX9 expression is frequently elevated in breast cancer (BC) cells and tissues, which suggests poor survival. The viability and motility of BC cells are irritated by enhanced DHX9 expression.
Large-Scale Characterization of Orthotopic Cell Line–Derived Xenografts Identifies TGFβ Signaling as a Key Regulator of Breast Cancer Morphology and Aggressiveness
[Cancer Research] The authors generated orthotopic cell line–derived xenograft (CDX) models from breast cancer cell lines using both mammary intraductal injections and fat pad transplantations. The resulting mammary intraductal CDX and fat pad transplantation CDX models covered the full spectrum of disease progression.
Metabolic Hallmarks of Trastuzumab Resistance
[Expert Opinion on Therapeutic Targets] The authors review the metabolic strategies that HER2+ breast cancer cells adopt to enable trastuzumab resistance, grouping them into a structured classification that takes into account their functional nature, namely: (1) metabolic reprogramming; (2) adaptive stress response; and (3) metabolic-signaling crosstalk.
Gut Microbial-Derived N-Acetylmuramic Acid Alleviates Colorectal Cancer via the AKT1 Pathway
[Gut] The effects of peptidoglycan fragments on intestinal tumourigenesis were systematically evaluated using various murine models of colorectal cancer (CRC) and organoids derived from CRC patients.
Establishment of a Cochlear Organoid Platform for Remodeling the Extracellular Matrix
[ACS Nano] Investigators developed a cochlear organoid culture system using synthetic GelMA hydrogel, which promoted the spontaneous clustering and organization of cochlear stem cells, thereby accelerating organoid formation and facilitating the functional maturation of cochlear hair cells.
Immunogenicity of Autologous and Allogeneic Human Primary Cholangiocyte Organoid Cellular Therapies
[Cell Reports Medicine] Scientists used in vitro co-cultures and in vivo humanized mouse experimental models to examine autologous and allogeneic immune response to human primary cholangiocyte organoids as treatment for bile duct disorders.
SARS-CoV-2 Induces Alzheimer’S Disease-Related amyloid-β Pathology in Ex Vivo Human Retinal Explants and Retinal Organoids
[Science Advances] To understand the association of amyloid-β plaques and pathogenic infections in the central nervous system, scientists obtained viable short-interval postmortem human retinal tissue and generated human retinal organoids that contain electrophysiologically active neurons.