Chemical Combinations Potentiate Human Pluripotent Stem Cell-Derived 3D Pancreatic Progenitor Clusters toward Functional β Cells

To efficiently cluster hPSC-derived pancreatic progenitors into 3D structures, scientists discovered 10 chemicals that not only retained the pancreatic progenitors in 3D clusters but also enhanced their potentiality towards NKX6.1+/INS+β cells.
[Nature Communications]
Full Article
Bookmark

No account yet? Register

0
Share

NR4A1 Enhances MKP7 Expression to Diminish JNK Activation Induced by ROS or ER-Stress in Pancreatic β Cells for Surviving

To search other possible mechanisms, scientists found the mRNA level and protein level of MKP7 (a phosphatase for phospho-JNK) were dramatically reduced in pancreatic β cells in the islets from NR4A1 knockout mice compared with that from wild type mice.
[Cell Death & Disease]
Pu, Z., Yu, T., Liu, D., Jin, C., Sadiq, E., Qiao, X., Li, X., Chen, Y., Zhang, J., Tian, M., Li, S., Zhao, R., & Wang, X. (2021). NR4A1 enhances MKP7 expression to diminish JNK activation induced by ROS or ER-stress in pancreatic β cells for surviving. Cell Death Discovery, 7(1), 1–13. https://doi.org/10.1038/s41420-021-00521-0 Cite
Full Article
Bookmark

No account yet? Register

0
Share

Abnormal Expression of microRNA-296-3p in Type 2 Diabetes Patients and its Role in Pancreatic β-Cells Function by Targeting Tensin Homolog Deleted on Chromosome Ten

Min6 cells were induced by 5 mg/dl UA and the cell proliferation, apoptosis, and insulin release were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry and glucose-stimulated insulin secretion, respectively.
[Biochemical Genetics]
Cheng, M., Guo, Y., Zhong, W., Chen, X., & Guo, G. (2021). Abnormal Expression of microRNA-296-3p in Type 2 Diabetes Patients and its Role in Pancreatic β-Cells Function by Targeting Tensin Homolog Deleted on Chromosome Ten. Biochemical Genetics. https://doi.org/10.1007/s10528-021-10083-6 Cite
Abstract
Bookmark

No account yet? Register

0
Share

Glucose Treatment of Human Pancreatic β-Cells Enhances Translation of mRNAs Involved in Energetics and Insulin Secretion

Reserachers described and validated the use of the SUnSET approach to demonstrate through FACS and Western Blot that glucose increases protein translation in the human cell line EndoC-βH2
[Journal of Biological Chemistry]
Glucose treatment of human pancreatic β-cells enhances translation of mRNAs involved in energetics and insulin secretion. (2021). Journal of Biological Chemistry, 100839. https://doi.org/10.1016/j.jbc.2021.100839 Cite
Full Article
Bookmark

No account yet? Register

0
Share

Transcriptional Mechanisms of Pancreatic β-Cell Maturation and Functional Adaptation

Scientists review emerging evidence that developmental and homeostatic regulation of β-cell function involves collaboration between lineage-determining and signal-dependent transcription factors.
[Trends in Endocrinology and Metabolism]
Wortham, M., & Sander, M. (2021). Transcriptional mechanisms of pancreatic β-cell maturation and functional adaptation. Trends in Endocrinology & Metabolism, 0(0). https://doi.org/10.1016/j.tem.2021.04.011 Cite
Full Article
Bookmark

No account yet? Register

0
Share

Pancreatic Cell Fate Specification: Insights into Developmental Mechanisms and Their Application for Lineage Reprogramming

The authors review recent advances in the fields of pancreas development and lineage reprogramming.
[Current Opinion in Genetics & Development]
Pancreatic cell fate specification: insights into developmental mechanisms and their application for lineage reprogramming. (2021). Current Opinion in Genetics & Development, 70, 32–39. https://doi.org/10.1016/j.gde.2021.05.003 Cite
Abstract
Bookmark

No account yet? Register

0
Share

Live-Cell Imaging of Glucose-Induced Metabolic Coupling of β and α Cell Metabolism in Health and Type 2 Diabetes

Researchers used phasor – Fluorescence Lifetime Imaging Microscopy to monitor oxidative phosphorylation and glycolysis in living islet cells before and after glucose stimulation.
[Communications Biology]
Wang, Z., Gurlo, T., Matveyenko, A. V., Elashoff, D., Wang, P., Rosenberger, M., Junge, J. A., Stevens, R. C., White, K. L., Fraser, S. E., & Butler, P. C. (2021). Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes. Communications Biology, 4(1), 1–11. https://doi.org/10.1038/s42003-021-02113-1 Cite
Full Article
Bookmark

No account yet? Register

0
Share

In Vivo Screen Identifies a SIK Inhibitor That Induces β Cell Proliferation through a Transient UPR

Single-cell transcriptomic analyses of mouse β cells demonstrate that HG-9-91-01 induces a wave of activating transcription factor (ATF)6-dependent unfolded protein response (UPR) before cell cycle entry.
[Nature Metabolism]
Charbord, J., Ren, L., Sharma, R. B., Johansson, A., Ågren, R., Chu, L., Tworus, D., Schulz, N., Charbord, P., Stewart, A. F., Wang, P., Alonso, L. C., & Andersson, O. (2021). In vivo screen identifies a SIK inhibitor that induces β cell proliferation through a transient UPR. Nature Metabolism, 3(5), 682–700. https://doi.org/10.1038/s42255-021-00391-x Cite
Full Article
Bookmark

No account yet? Register

0
Share

A Selective Look at Autophagy in Pancreatic β-Cells

Researchers examined the unique molecular players underlying each distinct form of autophagy in β-cells, including selective autophagy of mitochondria, insulin granules, lipid, intracellular amyloid aggregates, endoplasmic reticulum, and peroxisomes.
[Diabetes]
Pearson, G. L., Gingerich, M. A., Walker, E. M., Biden, T. J., & Soleimanpour, S. A. (2021). A Selective Look at Autophagy in Pancreatic β-Cells. Diabetes. https://doi.org/10.2337/dbi20-0014 Cite
Abstract
Bookmark

No account yet? Register

0
Share

Glucose-Induced Insulin Secretion in Isolated Human Islets: Does It Truly Reflect β-Cell Function In Vivo?

The aims are not to discuss specificities of pathways controlling secretion but to compare qualitative and quantitative features of glucose-induced insulin secretion in isolated human islets and in living human subjects.
[Molecular Metabolism]
Glucose-induced insulin secretion in isolated human islets: Does it truly reflect β-cell function in vivo? (2021). Molecular Metabolism, 48, 101212. https://doi.org/10.1016/j.molmet.2021.101212 Cite
Full Article
Bookmark

No account yet? Register

0
Share

SARS-CoV-2 Infects Human Pancreatic β-Cells and Elicits β-Cell Impairment

Researchers found that the SARS-CoV-2 receptor, ACE2 and related entry factors were expressed in β-cells, with selectively high expression of NRP1.
[Cell Metabolism]
Wu, C.-T., Lidsky, P. V., Xiao, Y., Lee, I. T., Cheng, R., Nakayama, T., Jiang, S., Demeter, J., Bevacqua, R. J., Chang, C. A., Whitener, R. L., Stalder, A. K., Zhu, B., Chen, H., Goltsev, Y., Tzankov, A., Nayak, J. V., Nolan, G. P., Matter, M. S., … Jackson, P. K. (2021). SARS-CoV-2 infects human pancreatic β-cells and elicits β-cell impairment. Cell Metabolism, 0(0). https://doi.org/10.1016/j.cmet.2021.05.013 Cite
AbstractGraphical Abstract
Bookmark

No account yet? Register

0
Share
Share