Researchers investigated the effects of two different types of protein kinase C (PKC) activators, the isophthalate derivative HMI-1b11 and bryostatin-1, on cardiomyocyte hypertrophy and elucidated the role of cPKCs and nPKCs in endothelin-1-induced hypertrophy in vitro.
[Frontiers in Pharmacology]
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Pohjolainen, L., Easton, J., Solanki, R., Ruskoaho, H., & Talman, V. (2021). Pharmacological Protein Kinase C Modulators Reveal a Pro-hypertrophic Role for Novel Protein Kinase C Isoforms in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Frontiers in Pharmacology, 11. https://doi.org/10.3389/fphar.2020.553852 Cite
Deficiency of Vsnl1 not only reduced the beating rate of human iPSC- derived cardiomyocytes but also the heart rate of mice. Weighted gene co-expression network analysis unveiled the core gene regulation network governing the function of the sinoatrial node in mice.
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Liang, D., Xue, J., Geng, L., Zhou, L., Lv, B., Zeng, Q., Xiong, K., Zhou, H., Xie, D., Zhang, F., Liu, J., Liu, Y., Li, L., Yang, J., Xue, Z., & Chen, Y.-H. (2021). Cellular and molecular landscape of mammalian sinoatrial node revealed by single-cell RNA sequencing. Nature Communications, 12(1), 287. https://doi.org/10.1038/s41467-020-20448-x Cite
The authors reported that TEAD1 is required for postmitotic cardiomyocyte (CM) survival. They found that adult mice with ubiquitous or CM-specific loss of Tead1 present with a rapid lethality due to an acute-onset dilated cardiomyopathy.
[Cell Death & Differentiation]
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Liu, J., Wen, T., Dong, K., He, X., Zhou, H., Shen, J., Fu, Z., Hu, G., Ma, W., Li, J., Wang, W., Wang, L., Akerberg, B. N., Xu, J., Osman, I., Zheng, Z., Wang, W., Du, Q., Pu, W. T., … Zhou, J. (2021). TEAD1 protects against necroptosis in postmitotic cardiomyocytes through regulation of nuclear DNA-encoded mitochondrial genes. Cell Death & Differentiation, 1–15. https://doi.org/10.1038/s41418-020-00732-5 Cite
Neonatal rat cardiomyocytes and cardiac fibroblasts were used to validate the protective effects of 6-gingerol in response to phenylephrine and transforming growth factor-β challenge.
[Acta Pharmacologica Sinica]
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Ma, S., Guo, Z., Liu, F., Hasan, S.-G., Yang, D., Tang, N., An, P., Wang, M., Wu, H., Yang, Z., Fan, D., & Tang, Q. (2021). 6-Gingerol protects against cardiac remodeling by inhibiting the p38 mitogen-activated protein kinase pathway. Acta Pharmacologica Sinica, 1–12. https://doi.org/10.1038/s41401-020-00587-z Cite
Investigators developed a simple culture protocol under which the adult murine inguinal adipose tissue-derived stromal vascular fraction (Ad-SVF) reproducibly transdifferentiates into beating cardiomyocyte (CMs) without induction. The beating CMs showed the striated ventricular phenotype of cardiomyocytes and synchronised oscillation of the intracellular calcium concentration among cells on day 28 of Ad-SVF primary culture.
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Takashima, S., Usui, S., Inoue, O., Goten, C., Yamaguchi, K., Takeda, Y., Cui, S., Sakai, Y., Hayashi, K., Sakata, K., Kawashiri, M., & Takamura, M. (2021). Myocyte-specific enhancer factor 2c triggers transdifferentiation of adipose tissue-derived stromal cells into spontaneously beating cardiomyocyte-like cells. Scientific Reports, 11(1), 1520. https://doi.org/10.1038/s41598-020-80848-3 Cite
Using the rat embryonic stem cells model, investigators showed that ERG1 was crucial in cardiomyocyte lineage commitment via interactions with integrin β1.
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Researchers hypothesized that modulation of oxygen conditions during uman amniotic fluid stem cell generation enriches soluble bioactive factors and confers enhanced regenerative and cardioprotective effects on cardiovascular cells.
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Kukumberg, M., Phermthai, T., Wichitwiengrat, S., Wang, X., Arjunan, S., Chong, S. Y., Fong, C.-Y., Wang, J.-W., Rufaihah, A. J., & Mattar, C. N. Z. (2021). Hypoxia-induced amniotic fluid stem cell secretome augments cardiomyocyte proliferation and enhances cardioprotective effects under hypoxic-ischemic conditions. Scientific Reports, 11(1), 163. https://doi.org/10.1038/s41598-020-80326-w Cite
A new approach for recording intracellular signals of outstanding quality and with noninvasiveness is introduced. By taking profit of the concept of mirror charge in classical electrodynamics, the new proposed device transduces cell ionic currents into mirror charges in a microfluidic chamber, thus realizing a virtual mirror cell.
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Amniotic fluid stem cells (AFSCs), a relatively novel source of stem cells, have been exploited for their ability of pluripotent differentiation. In addition, since AFSCs are weakly positive for the major histocompatibility class II molecules, they may have high immune tolerance.
[International Journal of Molecular Sciences]
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Researchers propose using human-iPSC-derived cardiomyocytes/ cardiac-stromal progenitor cells and cardiac organoid cultures as innovative experimental model systems to mimic clinical treatment, resulting in reproducible data, and promote the identification of immunotherapy-induced biomarkers of both early and late cardiotoxicity.
By employing the human embryonic stem cell (hESC) to cardiomyocyte differentiation system and generating QKI-deficient hESCs using CRISPR/Cas9 gene editing technology, scientists analyzed the physiological role of QKI in cardiomyocyte differentiation, maturation, and contractile function.
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Chen, X., Liu, Y., Xu, C., Ba, L., Liu, Z., Li, X., Huang, J., Simpson, E., Gao, H., Cao, D., Sheng, W., Qi, H., Ji, H., Sanderson, M., Cai, C.-L., Li, X., Yang, L., Na, J., Yamamura, K., … Sun, N. (2021). QKI is a critical pre-mRNA alternative splicing regulator of cardiac myofibrillogenesis and contractile function. Nature Communications, 12(1), 89. https://doi.org/10.1038/s41467-020-20327-5 Cite