BRPF3-HUWE1-Mediated Regulation of MYST2 Is Required for Differentiation and Cell-Cycle Progression in Embryonic Stem Cells

Researchers provided novel insights into understanding the functions of Brpf3 in proper differentiation as well as cell-cycle progression of ESCs via regulation of Myst2 stability by obstructing Huwe1-mediated ubiquitination.
[Cell Death & Differentiation]
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High-Throughput Intracellular Biopsy of microRNAs for Dissecting the Temporal Dynamics of Cellular Heterogeneity

Using intracellular biopsy technique (inCell-Biopsy), scientists analyzed the temporal miRNA transcriptome over the differentiation of ESCs. The combinatorial miRNA expression patterns derived by inCell-Biopsy identified emerging cell subpopulations differentiated from ESCs and revealed the dynamic evolution of cellular heterogeneity.
[Science Advances]
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A lncRNA-miRNA-mRNA Network for Human Primed, Naive and Extended Pluripotent Stem Cells

Researchers successfully converted three in-house-derived primed human PSC lines (H10, H24, and iPS) to a naive state and an expanded pluripotent stem cell (EPS) state. Primed, naive and EPS cells displayed state-specific morphologies and expressed pluripotent markers.
[PLoS One]
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An In Vitro Model of Early Anteroposterior Organization during Human Development

The authors showed that human ESCs can be used to generate gastruloids—three-dimensional multicellular aggregates that differentiate to form derivatives of the three germ layers organized spatiotemporally, without additional extra-embryonic tissues.
[Nature]
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Impact of α-Synuclein Pathology on Transplanted hESC-Derived Dopaminergic Neurons in a Humanized α-Synuclein Rat Model of PD

Researchers established a humanized transplantation model of Parkinson’s disease that better recapitulates the main disease features, obtained by coinjection of preformed human α-synuclein (α-syn) fibrils and adeno-associated virus expressing human wild-type α-syn unilaterally into the rat substantia nigra.
[Proceedings of the National Academy of Sciences of the United States of America]
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Immunity-and-Matrix-Regulatory Cells Derived from Human Embryonic Stem Cells Safely and Effectively Treat Mouse Lung Injury and Fibrosis

Intravenous delivery of immunity- and matrix-regulatory cells, derived from human embryonic stem cells, inhibited both pulmonary inflammation and fibrosis in mouse models of lung injury, and significantly improved the survival rate of the recipient mice in a dose-dependent manner, likely through paracrine regulatory mechanisms.
[Cell Research]
Wu, J., Song, D., Li, Z., Guo, B., Xiao, Y., Liu, W., Liang, L., Feng, C., Gao, T., Chen, Y., Li, Y., Wang, Z., Wen, J., Yang, S., Liu, P., Wang, L., Wang, Y., Peng, L., Stacey, G. N., … Hao, J. (2020). Immunity-and-matrix-regulatory cells derived from human embryonic stem cells safely and effectively treat mouse lung injury and fibrosis. Cell Research, 1–16. https://doi.org/10.1038/s41422-020-0354-1 Cite
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TETs Compete with DNMT3 Activity in Pluripotent Cells at Thousands of Methylated Somatic Enhancers

Investigators analyzed the independent and combined effects of positive DNMT3A/B and negative TET1-3 regulators on the DNA methylation landscape using a panel of knockout human ESC lines.
[Nature Genetics]
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Hippo-Yap Signaling Controls Lineage Differentiation of Mouse Embryonic Stem Cells through Modulating the Formation of Super-Enhancers

Investigators found that knockout of Mst1 and Mst2, two key components of the Hippo signaling in mouse ESCs, resulted in a disruption of differentiation into mesendoderm lineage. They revealed a novel mechanism on how the Hippo-YAP signaling pathway dictated ESC lineage differentiation.
[Nucleic Acids Research]
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