TCF/LEF Regulation of the Topologically Associated Domain ADI Promotes mESCs to Exit the Pluripotent Ground State

The authors found that genetic deletion of the four genes encoding the TCF/LEF transcription factors confered mouse ESCs (mESCs) with the ability to self-renew in N2B27 medium alone, identifying TCF/LEF effectors that mediated exit from the pluripotent state.
[Cell Reports]
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The Combined Action of Esrrb and Nr5a2 Is Essential for Murine Naïve Pluripotency

Researchers reported that the conjunct activity of two orphan nuclear receptors, Esrrb and Nr5a2, paralleled the importance of that of Oct4 and Sox2 in naïve mouse ESCs. By occupying a large common set of regulatory elements, these two factors controlled the binding of Oct4, Sox2 and Nanog to DNA.
[Development]
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Blastocyst-Inspired Hydrogels to Maintain Undifferentiation of Mouse Embryonic Stem Cells

Investigators developed hydrogels that enabledmouse ESCs (mESCs) to maintain stemness in vitro in the absence of both leukemia inhibitory factor and mouse embryonic fibroblasts, two critical factors in the standard protocol for mESC maintenance.
[ACS Nano]
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A Microfluidic Approach to Rescue ALS Motor Neuron Degeneration Using Rapamycin

Researchers presented a transgenic, TDP-43-A315T, mouse model expressing an amyotrophic lateral sclerosis phenotype and demonstrate the presence of ubiquitinated cytoplasmic TDP-43 aggregates with > 80% cell death by 28 days post differentiation in vitro.
[Scientific Reports]
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Simple Derivation of Skeletal Muscle from Human Pluripotent Stem Cells Using Temperature-Sensitive Sendai Virus Vector

Investigators established a simple method to convert human pluripotent stem cells into skeletal muscle cells by using temperature-sensitive Sendai virus vector encoding myoblast determination protein , a myogenic master transcription factor.
[Journal of Cellular and Molecular Medicine]
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Efficient Biallelic Knock-In in Mouse Embryonic Stem Cells by In Vivo-Linearization of Donor and Transient Inhibition of DNA Polymerase θ/DNA-PK

Researchers optimized an homology-directed repair (HDR)-mediated knock-in method for mouse ESCs by improving the efficiency of HDR-mediated knock-in of a plasmid donor while almost completely suppressing non-homologous end joining/microhomology-mediated end joining-based integration.
[Scientific Reports]
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A Microfluidic Approach to Rescue ALS Motor Neuron Degeneration Using Rapamycin

Scientists presented a transgenic, TAR DNA-binding protein-43 (TDP-43)-A315T, mouse model expressing an Amyotrophic Lateral Sclerosis (ALS) phenotype and demonstrated the presence of ubiquitinated cytoplasmic TDP-43 aggregates with > 80% cell death by 28 days post differentiation in vitro.
[Scientific Reports]
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Pluripotent Stem Cells for Skeletal Tissue Engineering

Investigators review the use of human PSCs for skeletal tissue engineering, the different methods for characterization of skeletal cells and constructs at the tissue and single-cell level, and indicate newer resources not yet fully utilized in this field.
[Critical Reviews in Biotechnology]
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Microvessels Support Engraftment and Functionality of Human Islets and hESC-Derived Pancreatic Progenitors in Diabetes Models

Scientists enhanced subcutaneous engraftment by employing a unique vascularization strategy based on ready-made microvessels isolated from the adipose tissue.
[Cell Stem Cell]
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Tracking of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells in Premature Ovarian Failure Model Mice

The authors evaluated the migration and distribution of embryonic stem cell-derived MSCs in a model of chemotherapy-induced premature ovarian failure.
[Biochemical and Biophysical Research Communications]
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In Vitro Reconstitution of the Whole Male Germ-Cell Development from Mouse Pluripotent Stem Cells

Investigators showed an in vitro reconstitution of whole male germ-cell development by PSCs. Mouse ESCs were induced into primordial germ cell-like cells, which were expanded for epigenetic reprogramming.
[Cell Stem Cell]
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