embryonic stem cells

[Neuroscience Bulletin] To investigate whether the autism spectrum disorder-associated CHD7 intronic variant affects neural development, researchers established human embryonic stem cells carrying this variant using CRISPR/Cas9 methods and found that the level of CHD7 mRNA significantly decreased compared to control.

[Stem Cells] Scientists identify two Yap splicing isoforms, which showed equal expression levels but heterogeneous distribution in ESCs.

[EMBO Journal] Scientists identified the mechanism of this barrier and report that the histone deacetylase Hdac3 and the transcriptional corepressor Dax1 cooperatively limited the lineage repertoire of mESCs by silencing an enhancer of the extraembryonic endoderm‐specifying transcription factor Gata6.

[Scientific Reports] Scientists produced well-defined 2D and 2.5D microenvironments and monitored cell division rate and subcellular yes-associated protein localization in single mouse embryonic stem cells hence excluding cell–cell interactions.

[Cellular and Molecular Life Sciences] Scientists review which transcription factors and signaling pathways play a role in both maintenance of pluripotency as well as cell cycle progression.

[Biology of Reproduction] Scientists describe a series of experiments to determine whether derivation of ESC could be generated by replacing IWR-1 with other inhibitors of WNT signaling.