Using Induced Pluripotent Stem Cells to Investigate Human Neuronal Phenotypes in 1q21.1 Deletion and Duplication Syndrome

To determine the impact of copy number variation at the 1q21.1 locus on neuronal development, researchers generated iPSCs from individuals harbouring 1q21.1 deletion or duplication and differentiated them into functional cortical neurons.
[Molecular Psychiatry]
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Porcine Primordial Germ Cell-Like Cells Generated from Induced Pluripotent Stem Cells under Different Culture Conditions

The authors analyzed the influence of the in vitro environment on the induction of porcine iPSCs differentiation into primordial germ cell-like cells, providing a suitable in vitro model for use in translational studies and to help answer numerous remaining questions about germ cells.
[Stem Cell Reviews and Reports]
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A Human Isogenic iPSC-Derived Cell Line Panel Identifies Major Regulators of Aberrant Astrocyte Proliferation in Down Syndrome

Scientists established a human isogenic cell line panel based on Down syndrome-specific iPSCs, the XIST-mediated transcriptional silencing system in trisomic chromosome 21, and genome/chromosome-editing technologies to eliminate phenotypic fluctuations caused by genetic variation.
[Communications Biology]
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Biological Importance of OCT Transcription Factors in Reprogramming and Development

Investigators discuss the roles of the structural components of Oct proteins in reprogramming and how donor cell epigenomes endow Oct proteins with different reprogramming competences.
[Experimental & Molecular Medicine]
Kim, K.-P., Han, D. W., Kim, J., & Schöler, H. R. (2021). Biological importance of OCT transcription factors in reprogramming and development. Experimental & Molecular Medicine, 1–11. https://doi.org/10.1038/s12276-021-00637-4 Cite
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Induced Pluripotent Stem Cells (iPSCs) Provide a Potentially Unlimited T Cell Source for CAR-T Cell Development and Off-the-Shelf Products

Scientists discuss the progress made in induced pluripotent stem cells (iPSC)-derived T cells and provides a roadmap for the development of CAR iPSC-derived T cells and off-the-shelf T-iPSCs.
[Pharmaceutical Research]
Sadeqi Nezhad, M., Abdollahpour-Alitappeh, M., Rezaei, B., Yazdanifar, M., & Seifalian, A. M. (2021). Induced Pluripotent Stem Cells (iPSCs) Provide a Potentially Unlimited T Cell Source for CAR-T Cell Development and Off-the-Shelf Products. Pharmaceutical Research. https://doi.org/10.1007/s11095-021-03067-z Cite
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Bayer to Advance Two First-of-Its-Kind Investigational Cell and Gene Therapies for Parkinson’s Disease

Bayer AG announced that BlueRock Therapeutics successfully administered the first dose of its investigational pluripotent stem cell-derived dopaminergic neurons, named DA01, to a Parkinson’s disease patient in their open-label Phase I clinical safety and tolerability study.
[Bayer AG (BusinessWire, Inc.)]
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AP-1 Is a Temporally Regulated Dual Gatekeeper of Reprogramming to Pluripotency

Scientists examined epigenome remodeling at the onset of human nuclear reprogramming by profiling human fibroblasts after fusion with murine ESCs. Efficient reprogramming of human fibroblasts to iPSCs was achieved by transduction with vectors expressing SOX2, KLF4, and inducible dnAP-1, demonstrating that dnAP-1 can substitute for exogenous human OCT4.
[Proceedings of the National Academy of Sciences of the United States of America]
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Contiguous Erosion of the Inactive X in Human Pluripotency Concludes with Global DNA Hypomethylation

Researchers performed an integrated analysis of DNA methylation, chromatin accessibility, and gene expression across hundreds of human PSC samples.
[Cell Reports]
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Deletion of lncRNA XACT Does Not Change Expression Dosage of X-linked Genes, but Affects Differentiation Potential in human PSCs

Scientists reported that a common mechanism for the initiation of the erosion of X chromosome inactivation of female human PSCs depended on XIST loss but not XACT accumulation on inactive X chromosomes.
[Cell Reports]
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Characterization of a Commercially Available Line of iPSC Hepatocytes as Models of Hepatocyte Function and Toxicity for Regulatory Purposes

The authors assessed similarities and differences between commercially available iPSC hepatocytes and primary human hepatocytes in preliminary assays of drug metabolism, hepatotoxicity, and drug transport.
[Journal of Pharmacological and Toxicological Methods]
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An Overview on Promising Somatic Cell Sources Utilized for the Efficient Generation of Induced Pluripotent Stem Cells

Scientists provide an overview of promising easily accessible somatic cell sources isolated in non-invasive or minimally invasive manner such as keratinocytes, urine cells, and peripheral blood mononuclear cells used for the generation of human iPSCs derived from healthy and diseased subjects.
[Stem Cell Reviews and Reports]
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