induced pluripotent stem cells

[CRISPR Journal] The authors summarize the increasingly refined and diverse CRISPR toolkit for the latter forms of gene perturbation in human PSCs and their downstream applications.

[Frontiers in Immunology] Scientists describe basic knowledge of T cell biology, applications in adoptive cell therapy, the use of iPSCs as a new source of T cells and current differentiation strategies used to generate T cells as well as recent advances in genome engineering to produce next-generation off-the-shelf T cells with improved effector functions.

[Stem Cell Reports] Sequencing of mitochondrial DNA (mtDNA) of 26 iPSC clones did not reveal evidence for de novo mutagenesis, or for any selection processes during reprogramming or differentiation. Culture expansion, however, selected against putatively actionable mtDNA mutations.

[Stem Cell Reports] Investigators demonstrated that microsatellite alterations were elevated in both mouse and human iPSCs, and identified a type of human iPSC in which these alterations were considerably reduced.

[Stem Cell Research & Therapy] The in vitro and in vivo cartilage and bone tissue formation potencies of human PSCs were investigated. The iPSCs were specified towards the mesodermal lineage and differentiated towards chondrocytes, which subsequently self-assembled into cartilaginous organoids.

[Proceedings of the National Academy of Sciences of the United States of America] Scientists generated retinal organoids from achromatopsia patient iPSCs carrying ATF6 disease variants and from gene-edited ATF6 null human ESCs, and found that achromatopsia patient and ATF6 null retinal organoids failed to form cone structures concomitant with loss of cone phototransduction gene expression, while rod photoreceptors developed normally.