induced pluripotent stem cells

[Cell Death & Disease] The exact role of SOX10 in human neural crest development and the underlying molecular mechanisms of SOX10-related human diseases remain poorly understood due to the lack of appropriate human model systems. Researchers successfully generated SOX10-knockout human induced pluripotent stem cells by the CRISPR-Cas9 gene editing tool.

[Stem Cell Reports] Scientists established a robust growth-factor-free protocol for generating definitive endoderm and pancreatic progenitors cells, facilitating scalable production of pancreatic cells for regenerative applications.

[Nature Communications] The authors reported a method to generate developmentally relevant human heart organoids by self-assembly using human PSCs. Heart organoids were comparable to age-matched human fetal cardiac tissues at the transcriptomic, structural, and cellular level.

[Scientific Reports] Scientists developed a homozygous NaV1.5 KO iPSC line able to differentiate into cardiomyocytes with CRISPR/Cas9 tool. The NaV1.5 KO iPSC-cardiomyocytes exhibited an organized contractile apparatus, spontaneous contractile activity, and electrophysiological recordings confirmed the major reduction in total Na+ currents.

[Nature Communications] Investigators employed a high-throughput cell aggregation approach to simultaneously coax mouse ESCs into hundreds of uniform epiblast-like aggregates in a solid matrix-free manner.

[Science Advances] After wrapping iPSC protein, the nanovaccine iPSC@RBC-Mlipo exhibited obvious splenic accumulation, systemic specific antitumor immunity evocation, and effective tumor expansion and metastasis inhibition in mice.