induced pluripotent stem cells

[Heliyon] Researchers mechanically cut 70-day-old human PSC-derived cerebral organoids (HCOs) with a scalpel blade into two to four pieces, each depending on their original size. After culturing cut hCOs for additional seven days, their size was less variable and smaller than uncut hCOs and there were no histological differences between uncut and cut hCOs.

[Nucleic Acids Research] Scientists examined the impact of Ino80 deletion in naïve and primed PSCs. They found that Ino80 deletion had minimal effect on self-renewal and gene expression in the naïve state, but led to cellular differentiation and de-repression of developmental genes in the transition toward and maintenance of the primed state.

[Tissue Engineering and Regenerative Medicine] The authors summarize the formation strategies and a wide range of applications in biomedicine of liver organoid.

[Stem Cell Reports] Single-cell RNA sequencing across ezh1 genotypes revealed a dropout of ezh1+/− cells among arterial endothelium associated with positive regulation of gene transcription.

[Leukemia] Scientists demonstrated that hiPSC-extracellular vesicles improved functional properties of cord blood-HSPCs critical for their hematopoietic capacity including metabolic, hematopoietic and clonogenic potential as well as survival, chemotactic response to stromal cell-derived factor 1 and adhesion to the model components of hematopoietic niche in vitro.

[American Journal of Physiology-Gastrointestinal and Liver Physiology] Scientists provide highlights on the development of organoid technology, and the use of this culture system to study the pathophysiology of specific mutations in the development of pancreatic and gastric cancer.