lentiviral transduction

[eLife] Researchers described a primary ex vivo hepatitis B virus (HBV)-infection model derived from healthy donor liver organoids after challenge with recombinant virus or HBV-infected patient serum.

[Molecular Therapy-Nucleic Acids] A new strategy to modify MSCs to express specific chemokine receptors using mRNA engineering was developed to evaluate the homing ability of MSCs and its therapeutic effects for inflammatory bowel disease.

[Gene Therapy] The authors investigated the pulmonary transplantation of macrophages transgenic for alpha-1 antitrypsin (AAT) as a potential therapy for AAT deficiency-associated lung pathology.

[Scientific Reports] The iPSCs were used to model JAK2-mutated MPNs. Normal iPSCs underwent lentiviral transduction to overexpress JAK2p.V617F or JAK2p.N542_E543del under a doxycycline-inducible system. The modified iPSCs were differentiated into erythroid cells.

[HGG Advances] The authors found that the mutant induced changes in cell morphology and activated SGK1, a serine/threonine kinase known to regulate cell proliferation and apoptosis, via non-canonical activation.

[Scientific Reports] Scientists present an efficient, lentiviral transduction protocol for delivery of CRISPR/Cas9 to macrophages derived from human iPSC with efficiencies close to 100%.