The constructed multiplex siRNAs effectively knocked down the expression of Smad4 and/or Smad1, Smad5, and Smad8 in MSCs, and inhibited all aspects of BMP9-induced osteogenic differentiation in bone marrow MSCs, including decreased expression of osteogenic regulators/markers, reduced osteogenic marker ALP activity, and diminished in vitro matrix mineralization and in vivo ectopic bone formation
[Molecular Therapy-Nucleic Acids]
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He, F., Ni, N., Zeng, Z., Wu, D., Feng, Y., Li, A. J., Luu, B., Li, A. F., Qin, K., Wang, E., Wang, X., Wu, X., Luo, H., Zhang, J., Zhang, M., Mao, Y., Pakvasa, M., Wagstaff, W., Zhang, Y., … Fan, J. (2020). FAMSi: A Synthetic Biology Approach to the Fast Assembly of Multiplex siRNAs for Silencing Gene Expression in Mammalian Cells. Molecular Therapy - Nucleic Acids, 0(0). https://doi.org/10.1016/j.omtn.2020.10.007 Cite
WJ-MSCs were encapsulated in 3D hydrogels derived from human fibrin or platelet lysate and the oxygen level was adjusted to physiological normoxia (5% O2).
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Biomimetic microenvironmental preconditioning enhance neuroprotective properties of human mesenchymal stem cells derived from Wharton’s Jelly (WJ-MSCs) | Scientific Reports. (n.d.). Retrieved October 13, 2020, from https://www.nature.com/articles/s41598-020-74066-0 Cite
Single-cell mRNA-sequencing analysis of the highly metabolically (hm) active cells from metastatic prostate cancer patients revealed that approximately 10% were canonical EpCAM+ hm-circulating stromal cells (CStCs), 3% were EpCAM− hm-CTCs with up-regulation of prostate-related genes, and 87% were hm-CStCs with profiles characteristic for cancer-associated fibroblasts, mesenchymal stem cells, and endothelial cells.
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The authors report that deleting Pinch1 in limb MSCs and Pinch2 globally (double knockout) in mice caused severe chondrodysplasia, while single mutant mice did not display marked defects.
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Scientists showed that Gli1+ MSCs, previously shown to contribute to myofibroblasts during scarring, promoted metaplastic differentiation of airway progenitors into KRT5+ basal cells.
[Nature Cell Biology]
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Cassandras, M., Wang, C., Kathiriya, J., Tsukui, T., Matatia, P., Matthay, M., Wolters, P., Molofsky, A., Sheppard, D., Chapman, H., & Peng, T. (2020). Gli1 + mesenchymal stromal cells form a pathological niche to promote airway progenitor metaplasia in the fibrotic lung. Nature Cell Biology, 1–12. https://doi.org/10.1038/s41556-020-00591-9 Cite
The effects of miR‐140‐5p on tissue‐to‐bone healing was investigated. Clone formation experiment, flow cytometry, Alizarin Red S Staining and Oil Red O Staining were performed to investigate the biological characteristics of mouse embryonic bone marrow MSCs C3H10T1/2.
[Cell Biochemistry and Function]
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Investigators exploited the DMP1 promoter as a predictive marker of MSC differentiation towards osteoblasts. Using CRISPR/Cas9 technology, they identified a distinctive change in fluorescence intensities of GFP knock‐in and osteoblast differentiation.
[Journal of Gene Medicine]
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Shahabipour, F., Oskuee, R. K., Shokrgozar, M. A., Naderi‐Meshkin, H., Goshayeshi, L., & Bonakdar, S. (n.d.). CRISPR/Cas9 mediated GFP-Human Dentin Matrix Protein 1 (DMP1) Promoter knock-in at the ROSA26 locus in mesenchymal stem cell for monitoring osteoblast differentiation. The Journal of Gene Medicine, n/a(n/a), e3288. https://doi.org/10.1002/jgm.3288 Cite
The authors give a brief description of MSCs, their sources and markers, and the different attempts that have been made towards achieving the goal of differentiating MSCs into neurons.
[Journal of Molecular Neuroscience]
Sorrento Therapeutics, Inc. announced that it has entered into an exclusive license agreement with Personalized Stem Cells, Inc. to acquire global rights to its adipose-derived MSCs for patients suffering from acute respiratory distress syndrome associated with COVID-19, which have been cleared for a Phase I clinical trial by the FDA.
[Sorrento Therapeutics, Inc.]
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To identify the active paracrine factors released by macrophages in response to stimulation by mesenchymal stem cell (MSC) conditioned media scientists used an antibody array; identifying increased expression of the angiogenesis-related proteins stromal cell-derived factor 1 and plasminogen activator inhibitor-1. Knockdown of either protein inhibited the ability of conditioned media derived from MSC paracrine factor stimulated macrophages to induce endothelial cell differentiation both in vitro and in vivo.
[American Journal of Physiology-Cell Physiology]
Researchers created human induced pluripotent stem cells (hiPSCs) from different MSC sources to evaluate the capacity of MSC-derived iPSCs to differentiate into any cell type of the human body and to serve as an alternative source for iPSC generation.
[Journal of Materials Chemistry B]
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Ababneh, N. A., Al-Kurdi, B., Barham, R., Ali, D., Sharar, N., Abuarqoub, D., Jafar, H., Salah, B., & Awidi, A. (2020). Derivation of three human induced pluripotent stem cell lines (JUCTCi014-A, JUCTCi015-A, JUCTCi016-A) from mesenchymal stem cells (MSCs) derived from bone marrow, adipose tissue and Wharton’s jelly samples. Stem Cell Research, 49, 102000. https://doi.org/10.1016/j.scr.2020.102000 Cite