FAMSi: A Synthetic Biology Approach to the Fast Assembly of Multiplex siRNAs for Silencing Gene Expression in Mammalian Cells

The constructed multiplex siRNAs effectively knocked down the expression of Smad4 and/or Smad1, Smad5, and Smad8 in MSCs, and inhibited all aspects of BMP9-induced osteogenic differentiation in bone marrow MSCs, including decreased expression of osteogenic regulators/markers, reduced osteogenic marker ALP activity, and diminished in vitro matrix mineralization and in vivo ectopic bone formation
[Molecular Therapy-Nucleic Acids]
He, F., Ni, N., Zeng, Z., Wu, D., Feng, Y., Li, A. J., Luu, B., Li, A. F., Qin, K., Wang, E., Wang, X., Wu, X., Luo, H., Zhang, J., Zhang, M., Mao, Y., Pakvasa, M., Wagstaff, W., Zhang, Y., … Fan, J. (2020). FAMSi: A Synthetic Biology Approach to the Fast Assembly of Multiplex siRNAs for Silencing Gene Expression in Mammalian Cells. Molecular Therapy - Nucleic Acids, 0(0). https://doi.org/10.1016/j.omtn.2020.10.007 Cite
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Biomimetic Microenvironmental Preconditioning Enhance Neuroprotective Properties of Human Mesenchymal Stem Cells Derived From Wharton’s Jelly (WJ-MSCs)

WJ-MSCs were encapsulated in 3D hydrogels derived from human fibrin or platelet lysate and the oxygen level was adjusted to physiological normoxia (5% O2).
[Scientific Reports]
Biomimetic microenvironmental preconditioning enhance neuroprotective properties of human mesenchymal stem cells derived from Wharton’s Jelly (WJ-MSCs) | Scientific Reports. (n.d.). Retrieved October 13, 2020, from https://www.nature.com/articles/s41598-020-74066-0 Cite
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Probing Single-Cell Metabolism Reveals Prognostic Value of Highly Metabolically Active Circulating Stromal Cells in Prostate Cancer

Single-cell mRNA-sequencing analysis of the highly metabolically (hm) active cells from metastatic prostate cancer patients revealed that approximately 10% were canonical EpCAM+ hm-circulating stromal cells (CStCs), 3% were EpCAM hm-CTCs with up-regulation of prostate-related genes, and 87% were hm-CStCs with profiles characteristic for cancer-associated fibroblasts, mesenchymal stem cells, and endothelial cells.
[Science Advances]
Rivello, F., Matuła, K., Piruska, A., Smits, M., Mehra, N., & Huck, W. T. S. (2020). Probing single-cell metabolism reveals prognostic value of highly metabolically active circulating stromal cells in prostate cancer. Science Advances, 6(40), eaaz3849. https://doi.org/10.1126/sciadv.aaz3849 Cite
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LIM Domain Proteins Pinch1/2 Regulate Chondrogenesis and Bone Mass in Mice

The authors report that deleting Pinch1 in limb MSCs and Pinch2 globally (double knockout) in mice caused severe chondrodysplasia, while single mutant mice did not display marked defects.
[Bone Research]
Lei, Y., Fu, X., Li, P., Lin, S., Yan, Q., Lai, Y., Liu, X., Wang, Y., Bai, X., Liu, C., Chen, D., Zou, X., Cao, X., Cao, H., & Xiao, G. (2020). LIM domain proteins Pinch1/2 regulate chondrogenesis and bone mass in mice. Bone Research, 8(1), 1–13. https://doi.org/10.1038/s41413-020-00108-y Cite
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Gli1+ Mesenchymal Stromal Cells Form a Pathological Niche to Promote Airway Progenitor Metaplasia in the Fibrotic Lung

Scientists showed that Gli1+ MSCs, previously shown to contribute to myofibroblasts during scarring, promoted metaplastic differentiation of airway progenitors into KRT5+ basal cells.
[Nature Cell Biology]
Cassandras, M., Wang, C., Kathiriya, J., Tsukui, T., Matatia, P., Matthay, M., Wolters, P., Molofsky, A., Sheppard, D., Chapman, H., & Peng, T. (2020). Gli1 + mesenchymal stromal cells form a pathological niche to promote airway progenitor metaplasia in the fibrotic lung. Nature Cell Biology, 1–12. https://doi.org/10.1038/s41556-020-00591-9 Cite
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MiR-140-5p Promotes Osteogenic Differentiation of Mouse Embryonic Bone Marrow Mesenchymal Stem Cells and Post-Fracture Healing of Mice

The effects of miR‐140‐5p on tissue‐to‐bone healing was investigated. Clone formation experiment, flow cytometry, Alizarin Red S Staining and Oil Red O Staining were performed to investigate the biological characteristics of mouse embryonic bone marrow MSCs C3H10T1/2.
[Cell Biochemistry and Function]
Jiao, J., Feng, G., Wu, M., Wang, Y., Li, R., & Liu, J. (n.d.). MiR-140-5p promotes osteogenic differentiation of mouse embryonic bone marrow mesenchymal stem cells and post-fracture healing of mice. Cell Biochemistry and Function, n/a(n/a). https://doi.org/10.1002/cbf.3585 Cite
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CRISPR/Cas9 Mediated GFP-Human Dentin Matrix Protein 1 (DMP1) Promoter Knock-In at the ROSA26 Locus in Mesenchymal Stem Cell for Monitoring Osteoblast Differentiation

Investigators exploited the DMP1 promoter as a predictive marker of MSC differentiation towards osteoblasts. Using CRISPR/Cas9 technology, they identified a distinctive change in fluorescence intensities of GFP knock‐in and osteoblast differentiation.
[Journal of Gene Medicine]
Shahabipour, F., Oskuee, R. K., Shokrgozar, M. A., Naderi‐Meshkin, H., Goshayeshi, L., & Bonakdar, S. (n.d.). CRISPR/Cas9 mediated GFP-Human Dentin Matrix Protein 1 (DMP1) Promoter knock-in at the ROSA26 locus in mesenchymal stem cell for monitoring osteoblast differentiation. The Journal of Gene Medicine, n/a(n/a), e3288. https://doi.org/10.1002/jgm.3288 Cite
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Therapeutic Advancement in Neuronal Transdifferentiation of Mesenchymal Stromal Cells for Neurological Disorders

The authors give a brief description of MSCs, their sources and markers, and the different attempts that have been made towards achieving the goal of differentiating MSCs into neurons.
[Journal of Molecular Neuroscience]
Choudhary, P., Gupta, A., & Singh, S. (2020). Therapeutic Advancement in Neuronal Transdifferentiation of Mesenchymal Stromal Cells for Neurological Disorders. Journal of Molecular Neuroscience. https://doi.org/10.1007/s12031-020-01714-5 Cite
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Sorrento Adds Mesenchymal Stem Cell Program (MSC), Cleared for Phase I Trial by the FDA to the Pipeline of COVID-19 Focused Rescue Therapies

Sorrento Therapeutics, Inc. announced that it has entered into an exclusive license agreement with Personalized Stem Cells, Inc. to acquire global rights to its adipose-derived MSCs for patients suffering from acute respiratory distress syndrome associated with COVID-19, which have been cleared for a Phase I clinical trial by the FDA.
[Sorrento Therapeutics, Inc.]
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Sequential Paracrine Mechanisms Are Necessary for the Therapeutic Benefits of Stem Cell Therapy

To identify the active paracrine factors released by macrophages in response to stimulation by mesenchymal stem cell (MSC) conditioned media scientists used an antibody array; identifying increased expression of the angiogenesis-related proteins stromal cell-derived factor 1 and plasminogen activator inhibitor-1. Knockdown of either protein inhibited the ability of conditioned media derived from MSC paracrine factor stimulated macrophages to induce endothelial cell differentiation both in vitro and in vivo.
[American Journal of Physiology-Cell Physiology]
Sun, H., Pratt, R. E., Hodgkinson, C. P., & Dzau, V. J. (2020). Sequential paracrine mechanisms are necessary for the therapeutic benefits of stem cell therapy. American Journal of Physiology-Cell Physiology. https://doi.org/10.1152/ajpcell.00516.2019 Cite
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Derivation of Three Human Induced Pluripotent Stem Cell Lines (JUCTCi014-A, JUCTCi015-A, JUCTCi016-a) from Mesenchymal Stem Cells (MSCs) Derived from Bone Marrow, Adipose Tissue and Wharton’s Jelly Samples

Researchers created human induced pluripotent stem cells (hiPSCs) from different MSC sources to evaluate the capacity of MSC-derived iPSCs to differentiate into any cell type of the human body and to serve as an alternative source for iPSC generation.
[Journal of Materials Chemistry B]
Ababneh, N. A., Al-Kurdi, B., Barham, R., Ali, D., Sharar, N., Abuarqoub, D., Jafar, H., Salah, B., & Awidi, A. (2020). Derivation of three human induced pluripotent stem cell lines (JUCTCi014-A, JUCTCi015-A, JUCTCi016-A) from mesenchymal stem cells (MSCs) derived from bone marrow, adipose tissue and Wharton’s jelly samples. Stem Cell Research, 49, 102000. https://doi.org/10.1016/j.scr.2020.102000 Cite
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