Prenatal Transplantation of Human Amniotic Fluid Stem Cell Could Improve Clinical Outcome of Type III Spinal Muscular Atrophy in Mice

Motor function was analyzed by three behavioral tests. Engraftment of human amniotic fluid stem cell in organs were assessed by flow cytometry and RNA scope.
[Scientific Reports]
Shaw, S. W., Peng, S.-Y., Liang, C.-C., Lin, T.-Y., Cheng, P.-J., Hsieh, T.-T., Chuang, H.-Y., De Coppi, P., & David, A. L. (2021). Prenatal transplantation of human amniotic fluid stem cell could improve clinical outcome of type III spinal muscular atrophy in mice. Scientific Reports, 11(1), 9158. https://doi.org/10.1038/s41598-021-88559-z Cite
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In Vitro Model of Ischemic Heart Failure Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

hiPSCs were differentiated to functional hiPSC derived cardiomyocytes (hiPSC-CMs) and then purified using either a simulated ischemia media or by using magnetic antibody-based purification targeting the non-myocyte population for depletion from the cell population. Flow cytometry analysis confirmed that each purification approach generated hiPSC-CM cultures of >94% cTnT+ cells.
[JCI Insight]
Davis, J., Chouman, A., Creech, J., Rocha, A. M. da, Ponce-Balbuena, D., Vazquez, E. N. J., Nichols, R., Lozhkin, A., Madamanchi, N. R., Campbell, K. F., & Herron, T. J. (2021). In vitro model of ischemic heart failure using human induced pluripotent stem cell-derived cardiomyocytes. JCI Insight. https://doi.org/10.1172/jci.insight.134368 Cite
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Valdecoxib Improves Lipid-Induced Skeletal Muscle Insulin Resistance via Simultaneous Suppression of Inflammation and Endoplasmic Reticulum Stress

Scientists suggest that valdecoxib alleviated insulin resistance through AMP-activated protein kinase/heat shock protein beta 1-mediated inhibition of inflammation and endoplasmic reticulum stress in skeletal muscle under hyperlipidemic conditions.
[Biochemical Pharmacology]
Kim, T. J., Lee, H. J., Pyun, D. H., Abd El-Aty, A. M., Jeong, J. H., & Jung, T. W. (2021). Valdecoxib improves lipid-induced skeletal muscle insulin resistance via simultaneous suppression of inflammation and endoplasmic reticulum stress. Biochemical Pharmacology, 188, 114557. https://doi.org/10.1016/j.bcp.2021.114557 Cite
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Isolation and Characterization of hESC-Derived Heart Field-Specific Cardiomyocytes Unravels New Insights into Their Transcriptional and Electrophysiological Profiles

Scientists described a high yield approach for isolation and characterization of hESC-derived heart field specific and nodal-like cardiomyocytes.
[Cardiovascular Research]
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Single Cell RNA-Sequencing Uncovers Paracrine Functions of the Epicardial-Derived Cells in Arrhythmogenic Cardiomyopathy

Epicardial-derived cardiac fibroblasts and epithelial cells express paracrine factors, including TGFβ1 and FGFs, which mediate EMT, and contribute to the pathogenesis of myocardial fibrosis, apoptosis, arrhythmias, and cardiac dysfunction in a mouse model of arrhythmogenic cardiomyopathy.
[Circulation]
Yuan Ping, Cheedipudi Sirisha M., Rouhi Leila, Fan Siyang, Simon Lukas, Zhao Zhongming, Hong Kui, Gurha Priyatansh, & Marian Ali J. (n.d.). Single Cell RNA-Sequencing Uncovers Paracrine Functions of the Epicardial-Derived Cells in Arrhythmogenic Cardiomyopathy. Circulation, 0(0). https://doi.org/10.1161/CIRCULATIONAHA.120.052928 Cite
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Temporal Mechanisms of Myogenic Specification in Human Induced Pluripotent Stem Cells

Scientists performed a longitudinal comparison of the transcriptomic profiles of three hiPSC lines that displayed differential myogenic specification, one robust and two blunted.
[Science Advances]
Nayak, P., Colas, A., Mercola, M., Varghese, S., & Subramaniam, S. (2021). Temporal mechanisms of myogenic specification in human induced pluripotent stem cells. Science Advances, 7(12), eabf7412. https://doi.org/10.1126/sciadv.abf7412 Cite
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Long Non-Coding RNA Mir22hg-Derived miR-22-3p Promotes Skeletal Muscle Differentiation and Regeneration by Inhibiting HDAC4

Researchers identified a novel lncRNA, Mir22hg, that is significantly up-regulated during myoblast differentiation and is highly expressed in skeletal muscle. They validated that Mir22hg promotes the myoblasts differentiation, in vitro.
[Molecular Therapy-Nucleic Acids]
Li, R., Li, B., Cao, Y., Li, W., Dai, W., Zhang, L., Zhang, X., Ning, C., Li, H., Yao, Y., Tao, J., Jia, C., Wu, W., & Liu, H. (2021). Long non-coding RNA Mir22hg-derived miR-22-3p promotes skeletal muscle differentiation and regeneration by inhibiting HDAC4. Molecular Therapy - Nucleic Acids, 0(0). https://doi.org/10.1016/j.omtn.2021.02.025 Cite
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Optogenetic Current in Myofibroblasts Acutely Alters Electrophysiology and Conduction of Co-Cultured Cardiomyocytes

Cardiac myofibroblasts were transduced with Channelrhodopsin-2, which allowed acute and selective increase of myofibroblast current, and plated on top of cardiomyocytes.
[Scientific Reports]
Kostecki, G. M., Shi, Y., Chen, C. S., Reich, D. H., Entcheva, E., & Tung, L. (2021). Optogenetic current in myofibroblasts acutely alters electrophysiology and conduction of co-cultured cardiomyocytes. Scientific Reports, 11(1), 4430. https://doi.org/10.1038/s41598-021-83398-4 Cite
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Exercise Triggers CAPN1-Mediated AIF Truncation, Inducing Myocyte Cell Death in Arrhythmogenic Cardiomyopathy

When cardiomyocytes differentiated from Dsg2mut/mut embryonic stem cells were challenged with β-adrenergic stimulation, calpain-1 (CAPN1) inhibition attenuated CAPN1-induced AIF truncation.
[Science Translational Medicine]
Chelko, S. P., Keceli, G., Carpi, A., Doti, N., Agrimi, J., Asimaki, A., Beti, C. B., Miyamoto, M., Amat-Codina, N., Bedja, D., Wei, A.-C., Murray, B., Tichnell, C., Kwon, C., Calkins, H., James, C. A., O’Rourke, B., Halushka, M. K., Melloni, E., … Paolocci, N. (2021). Exercise triggers CAPN1-mediated AIF truncation, inducing myocyte cell death in arrhythmogenic cardiomyopathy. Science Translational Medicine, 13(581). https://doi.org/10.1126/scitranslmed.abf0891 Cite
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Bioengineering the Extracellular Matrix to Improve the Cellular and Molecular Physiology of Cells In Vitro

The authors present a novel biomimetic substrate with submicron topographies that is capable of mimicking the mechanical and structural cues of the ECM while benefiting from high-precision, easy-to-reproduce, and scalable photolithography-based fabrication techniques.
[Biophysical Journal]

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MEF2C Shapes the Microtranscriptome during Differentiation of Skeletal Muscles

Deep sequencing of small RNAs of human muscle cells revealed a set of miRNAs, including several muscle-specific miRNAs, that were sensitive to myocyte enhancer factor 2C (MEF2C) depletion.
[Scientific Reports]
Piasecka, A., Sekrecki, M., Szcześniak, M. W., & Sobczak, K. (2021). MEF2C shapes the microtranscriptome during differentiation of skeletal muscles. Scientific Reports, 11(1), 3476. https://doi.org/10.1038/s41598-021-82706-2 Cite
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