Motor function was analyzed by three behavioral tests. Engraftment of human amniotic fluid stem cell in organs were assessed by flow cytometry and RNA scope.
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Shaw, S. W., Peng, S.-Y., Liang, C.-C., Lin, T.-Y., Cheng, P.-J., Hsieh, T.-T., Chuang, H.-Y., De Coppi, P., & David, A. L. (2021). Prenatal transplantation of human amniotic fluid stem cell could improve clinical outcome of type III spinal muscular atrophy in mice. Scientific Reports, 11(1), 9158. https://doi.org/10.1038/s41598-021-88559-z Cite
hiPSCs were differentiated to functional hiPSC derived cardiomyocytes (hiPSC-CMs) and then purified using either a simulated ischemia media or by using magnetic antibody-based purification targeting the non-myocyte population for depletion from the cell population. Flow cytometry analysis confirmed that each purification approach generated hiPSC-CM cultures of >94% cTnT+ cells.
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Davis, J., Chouman, A., Creech, J., Rocha, A. M. da, Ponce-Balbuena, D., Vazquez, E. N. J., Nichols, R., Lozhkin, A., Madamanchi, N. R., Campbell, K. F., & Herron, T. J. (2021). In vitro model of ischemic heart failure using human induced pluripotent stem cell-derived cardiomyocytes. JCI Insight. https://doi.org/10.1172/jci.insight.134368 Cite
Scientists suggest that valdecoxib alleviated insulin resistance through AMP-activated protein kinase/heat shock protein beta 1-mediated inhibition of inflammation and endoplasmic reticulum stress in skeletal muscle under hyperlipidemic conditions.
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Kim, T. J., Lee, H. J., Pyun, D. H., Abd El-Aty, A. M., Jeong, J. H., & Jung, T. W. (2021). Valdecoxib improves lipid-induced skeletal muscle insulin resistance via simultaneous suppression of inflammation and endoplasmic reticulum stress. Biochemical Pharmacology, 188, 114557. https://doi.org/10.1016/j.bcp.2021.114557 Cite
Scientists described a high yield approach for isolation and characterization of hESC-derived heart field specific and nodal-like cardiomyocytes.
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Epicardial-derived cardiac fibroblasts and epithelial cells express paracrine factors, including TGFβ1 and FGFs, which mediate EMT, and contribute to the pathogenesis of myocardial fibrosis, apoptosis, arrhythmias, and cardiac dysfunction in a mouse model of arrhythmogenic cardiomyopathy.
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Yuan Ping, Cheedipudi Sirisha M., Rouhi Leila, Fan Siyang, Simon Lukas, Zhao Zhongming, Hong Kui, Gurha Priyatansh, & Marian Ali J. (n.d.). Single Cell RNA-Sequencing Uncovers Paracrine Functions of the Epicardial-Derived Cells in Arrhythmogenic Cardiomyopathy. Circulation, 0(0). https://doi.org/10.1161/CIRCULATIONAHA.120.052928 Cite
Scientists performed a longitudinal comparison of the transcriptomic profiles of three hiPSC lines that displayed differential myogenic specification, one robust and two blunted.
Researchers identified a novel lncRNA, Mir22hg, that is significantly up-regulated during myoblast differentiation and is highly expressed in skeletal muscle. They validated that Mir22hg promotes the myoblasts differentiation, in vitro.
[Molecular Therapy-Nucleic Acids]
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Li, R., Li, B., Cao, Y., Li, W., Dai, W., Zhang, L., Zhang, X., Ning, C., Li, H., Yao, Y., Tao, J., Jia, C., Wu, W., & Liu, H. (2021). Long non-coding RNA Mir22hg-derived miR-22-3p promotes skeletal muscle differentiation and regeneration by inhibiting HDAC4. Molecular Therapy - Nucleic Acids, 0(0). https://doi.org/10.1016/j.omtn.2021.02.025 Cite
Cardiac myofibroblasts were transduced with Channelrhodopsin-2, which allowed acute and selective increase of myofibroblast current, and plated on top of cardiomyocytes.
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When cardiomyocytes differentiated from Dsg2mut/mut embryonic stem cells were challenged with β-adrenergic stimulation, calpain-1 (CAPN1) inhibition attenuated CAPN1-induced AIF truncation.
[Science Translational Medicine]
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Chelko, S. P., Keceli, G., Carpi, A., Doti, N., Agrimi, J., Asimaki, A., Beti, C. B., Miyamoto, M., Amat-Codina, N., Bedja, D., Wei, A.-C., Murray, B., Tichnell, C., Kwon, C., Calkins, H., James, C. A., O’Rourke, B., Halushka, M. K., Melloni, E., … Paolocci, N. (2021). Exercise triggers CAPN1-mediated AIF truncation, inducing myocyte cell death in arrhythmogenic cardiomyopathy. Science Translational Medicine, 13(581). https://doi.org/10.1126/scitranslmed.abf0891 Cite
The authors present a novel biomimetic substrate with submicron topographies that is capable of mimicking the mechanical and structural cues of the ECM while benefiting from high-precision, easy-to-reproduce, and scalable photolithography-based fabrication techniques.
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Deep sequencing of small RNAs of human muscle cells revealed a set of miRNAs, including several muscle-specific miRNAs, that were sensitive to myocyte enhancer factor 2C (MEF2C) depletion.